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Candida utilis strain and application of same in preparation of selenium-rich yeast products

A technology of Candida utilis and selenium-enriched yeast, applied in the biological field, can solve problems such as limited nutritional value, poor reproducibility, and weak selenium-enriched ability

Active Publication Date: 2012-12-12
XIAMEN YUANZUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been many studies on selenium-enriched yeast, but in most research subjects, the selenium-enrichment ability of the strains is relatively weak. Generally, the selenium content is between 500-1500mg / kg, and the biomass is also very low. It is difficult to Achieving high density quantities required for a large production with poor reproducibility
In addition, the form of selenium in the yeast is also mainly the inorganic selenium form adsorbed on the cell surface or mixed with the cells, the organic form of selenium, especially the content of selenomethionine is extremely low, and its nutritional value is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, acquisition and preservation of bacterial strain S1204

[0050] Using the strain Candida utilis 1268 as the starting strain (purchased from the China Industrial Microbiology Culture Collection Management Center, CICC No. 1268), through multiple physical-chemical compound mutation breeding and high-throughput screening methods Yeast strain S1204 with high biomass and high selenium conversion, the specific method is as follows:

[0051] 1. Combined mutagenesis treatment of UV (ultraviolet) mutagenesis and NTG (nitrosoguanidine) of the strain

[0052] Take 5 mL of Candida utilis 1268 bacterial solution in the logarithmic phase of growth, centrifuge at 4800r / min for 5min, wash with sterile water twice, add pH7.2 phosphate buffer to make bacterial suspension. Take the whole amount of the prepared bacterial suspension and place it in a sterile plate with a diameter of 9 cm, put a sterile magnetic stirring bar on it, place it on the magnetic stirrer, and preheat...

Embodiment 2

[0057] Example 2, the application of selenium-enriched yeast S1204 CCTCC NO:M2012216 in selenium-enrichment

[0058] 1. Optimization of culture medium formula

[0059] In the medium, wort juice is the main carbon source in the medium, and the highest biomass and selenium content can be obtained when the added sugar content is 90g / L-110g / L; peptone and yeast extract are the nitrogen sources in the medium; Sodium selenium is selected as the inorganic selenium source in the culture medium, the initial addition amount of seed medium is 50mg / L, the initial addition amount of fermentation medium is 100mg / L, and the sodium selenite concentration of flow-added selenium is 10g / L .

[0060] The specific formulation of the optimized medium is as follows:

[0061] The seed culture medium is prepared according to the following method: the wort with a sugar content of 90g / L, peptone, yeast extract and anhydrous sodium selenite are mixed to obtain a seed culture medium, the concentration o...

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Abstract

The invention discloses a Candida utilis strain and application of the same in preparation of selenium-rich yeast products. The accession number of Candida utilis S1204 provided by the invention is CCTCC No: M2012216. The application of the Candida utilis S1204 in preparation of selenium-rich yeast products is also in the scope of protection of the invention. According to results of experiments in the invention, a bred novel strain of the Candida utilis S1204 has strong capability in selenium transfer and selenium enrichment during high biomass fermentation and can be used for preparing selenium-rich yeast products.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Candida utilis and its application in preparing selenium-enriched yeast products. Background technique [0002] After the Swedish chemist Berzeliu first discovered the element selenium (selenium) in 1817, people have been studying selenium as a toxic element until 1957 when American scientist Sehwarz and others discovered the nutritional efficacy of selenium, which made people study selenium from a new perspective. In 1972, Roetueh confirmed that selenium is the active ingredient of glutathione peroxidase (GPX), so selenium is recognized as an essential trace element for life. In 1973, the World Health Organization announced that selenium is an essential trace element for humans. The Chinese Nutrition Society also listed selenium as an essential nutritional trace element in 1988. [0003] As an essential nutritional trace element for humans and animals, selenium partici...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/38A23K1/16C12R1/72
Inventor 黄钦耿倪村标孙艳蔡玉凤骆梅香朱穗贞刘建明施巧琴吴松刚
Owner XIAMEN YUANZUN BIOLOGICAL ENG CO LTD
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