Selenium-rich acremonium terricola strain and application thereof

A technology of Acremonium acremonium and Acremonium acremonium, which is applied to selenium-enriched Acremonium acremonium strains and its application field, and can solve the problems of low content of secondary metabolites such as cordycepin and the limitation of the utilization of Acremonium acremonium

Active Publication Date: 2016-09-28
FUJIAN NORMAL UNIV +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the content of active ingredients such as the secondary metabolite cordycepin in the currently discovered strains of Acremonium acremonium is not high, which limits the further utilization of Acremonium acremonium
Not only that, but at present, there are no reports on the preparation of selenium-enriched Acremonium strains and their cultures. How to realize the transformation and enrichment of selenium by the strains on the basis of increasing the main active ingredient of the Acremonium strains, cordycepin. It has become a new research topic to develop a new type of Acremonium acremonium culture product that integrates many physiological functions of Cordyceps fungus and selenium, which has significant innovative value and application in the further development of Acremonium acremonium and its culture. economic benefits

Method used

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  • Selenium-rich acremonium terricola strain and application thereof
  • Selenium-rich acremonium terricola strain and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0070] The preparation method of seed strengthening medium: 20g of soybean powder, 10g of sucrose, 5.0g of yeast extract, 3.0g of peptone, 0.5g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, 100mg of ferrous sulfate, vitamin B 1 20mg, vitamin B 2 10mg, vitamin B 6 10mg, vitamin B 12 50 μg, distilled water to 1000ml to make a nutrient solution; 1000ml nutrient solution was mixed with 1000g wheat evenly, steamed in water for 45min, and sterilized by high-pressure steam at 121°C for 20-30min to obtain a seed strengthening medium. The concentration of each solute in the nutrient solution is as follows: soybean powder 2g / 100ml, sucrose 1g / 100ml, yeast extract 0.5g / 100ml, peptone 0.3g / 100ml, potassium dihydrogen phosphate 0.05g / 100ml, magnesium sulfate 0.01g / 100ml, Ferrous Sulfate 10mg / 100ml, Vitamin B 1 2mg / 100ml, vitamin B 2 1mg / 100ml, vitamin B 6 1mg / 100ml, vitamin B 12 5 μg / 100ml.

[0071] The preparation method of the liquid fermentation medium: ta...

Embodiment 1

[0084] Embodiment 1, the breeding of Acremonium acremonium FA1603

[0085] 1. Collection of starting strain mycelium

[0086] 1. Using the Acremonium acremonium strain isolated and screened from wild Cordyceps militaris fruiting bodies in Changbai Mountain, Liaoning as the starting strain, the starting strain was inoculated into PDA solid slant medium, and cultured at 25° C. for 4 days.

[0087] 2. Elute the spores on the PDA solid slant medium cultivated in step 1 with sterile physiological saline, and filter through sterile filter paper to obtain a spore suspension, and the spore concentration in the spore suspension is 0.5×10 6 individual / mL.

[0088] 3. Transfer the spore suspension prepared in step 2 to a PDA liquid medium for culture (25° C., 200 rpm, 48 h) at a 5% inoculum size to obtain a liquid culture.

[0089] 4. Centrifuge the liquid culture obtained in step 3 at 6000 rpm for 30 minutes to collect mycelium, wash it twice with sterile physiological saline, and dry...

Embodiment 2

[0115] Embodiment 2, the application of Acremonium acremonium FA1603

[0116] 1. Seed intensive cultivation of Acremonium acremonium FA1603

[0117] 1. Inoculate Acremonium acremonium FA1603 onto a PDA solid medium slant (18×180 mm test tube), and culture at 25° C. for 5 days.

[0118] 2. Transfer the bacterial strain cultivated in step 1 to a PDA solid medium slant (18×180 mm test tube) containing 20 μg / mL sodium selenite, and culture at 25° C. for 3 days.

[0119] 3. The spores on the PDA solid medium slant obtained in step 2 were eluted with 20mL sterile physiological saline (the spore concentration in the eluate obtained was 0.5×10 5 individual / mL), all the eluate was transferred to an eggplant bottle containing 100 g seed strengthening medium containing 50 μg / mL sodium selenite, and cultivated in an incubator at 25 ° C for 3 days to obtain a strengthened seed culture ( The culture spore concentration can reach 1012 per gram of culture).

[0120] 2. Acremonium acremoniu...

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Abstract

The invention discloses a selenium-rich acremonium terricola strain and an application thereof. The collection number of the acremonium terricola FA1603 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2016331. The invention also provides an acremonium terricola culture and a method for culturing the acremonium terricola FA1603. In a selenium-rich acremonium terricola culture product with high cordycepin content produced by fermenting the acremonium terricola FA1603, the content of organic selenium is high while the organic selenium mainly consists of active selenomethionine; moreover, the acremonium terricola culture product is rich in cordyceps active ingredients, and particularly the cordycepin content is highlighted; in term of feed addition, the acremonium terricola culture product is safe and nontoxic without side effects, the animal meat nutrition can be remarkably improved, and the meat quality is enhanced; and meanwhile, the healthy development of animals is promoted, the body immunity is enhanced, and huge economic and social benefits are created.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a selenium-enriched Acremonium acremonium strain and application thereof. Background technique [0002] Traditionally, Cordyceps is a general term for all fungi of the genus Cordyceps sensu lato in the ergot family Clavicipitaceae, and is the fungus that forms "Cordyceps". At present, there are more than 500 names related to Cordyceps fungus reported and recorded in the world, and about 120 species have been reported in China. The host range of Cordyceps fungus is very wide, and can parasite animals (including insects, spiders, acarids and nematodes, etc.) or other fungi (such as fungi of the genus Elaphomyces Nees and the genus Claviceps Tul.). The complexes of some Cordyceps fungi and hosts can be directly used in traditional Chinese medicine, such as Cordyceps sinensis, Cordyceps militaris and cicadae, etc., and most of the Cordyceps fungi can also produce a variety of bi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K10/18A23L31/00C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 黄钦耿黄建忠蒋顺进方文棋吴松刚
Owner FUJIAN NORMAL UNIV
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