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A selenium-enriched Acremonium strain and its application

A technology of Acremonium acremonium and Acremonium, applied to selenium-enriched Acremonium acremonium strains and its application fields, which can solve the problems of limiting the use of Acremonium acremonium and the low content of active ingredients such as secondary metabolites cordycepin

Active Publication Date: 2019-03-26
FUJIAN NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of active ingredients such as the secondary metabolite cordycepin in the currently discovered strains of Acremonium acremonium is not high, which limits the further utilization of Acremonium acremonium
Not only that, but at present, there are no reports on the preparation of selenium-enriched Acremonium strains and their cultures. How to realize the transformation and enrichment of selenium by the strains on the basis of increasing the main active ingredient of the Acremonium strains, cordycepin. It has become a new research topic to develop a new type of Acremonium acremonium culture product that integrates many physiological functions of Cordyceps fungus and selenium, which has significant innovative value and application in the further development of Acremonium acremonium and its culture. economic benefits

Method used

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  • A selenium-enriched Acremonium strain and its application
  • A selenium-enriched Acremonium strain and its application

Examples

Experimental program
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preparation example Construction

[0070] The preparation method of seed strengthening medium: 20g of soybean powder, 10g of sucrose, 5.0g of yeast extract, 3.0g of peptone, 0.5g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, 100mg of ferrous sulfate, vitamin B 1 20mg, vitamin B 2 10mg, vitamin B 6 10mg, vitamin B 12 50 μg, distilled water to 1000ml to make a nutrient solution; 1000ml nutrient solution was mixed with 1000g wheat evenly, steamed in water for 45min, and sterilized by high-pressure steam at 121°C for 20-30min to obtain a seed strengthening medium. The concentration of each solute in the nutrient solution is as follows: soybean powder 2g / 100ml, sucrose 1g / 100ml, yeast extract 0.5g / 100ml, peptone 0.3g / 100ml, potassium dihydrogen phosphate 0.05g / 100ml, magnesium sulfate 0.01g / 100ml, Ferrous Sulfate 10mg / 100ml, Vitamin B 1 2mg / 100ml, vitamin B 2 1mg / 100ml, vitamin B 6 1mg / 100ml, vitamin B 12 5 μg / 100ml.

[0071] The preparation method of the liquid fermentation medium: tak...

Embodiment 1

[0084] Embodiment 1, the breeding of Acremonium acremonium FA1603

[0085] 1. Collection of starting strain mycelium

[0086] 1. Using the Acremonium acremonium strain isolated and screened from wild Cordyceps militaris fruiting bodies in Changbai Mountain, Liaoning as the starting strain, the starting strain was inoculated into PDA solid slant medium, and cultured at 25° C. for 4 days.

[0087] 2. Elute the spores on the PDA solid slant medium cultivated in step 1 with sterile physiological saline, and filter through sterile filter paper to obtain a spore suspension, and the spore concentration in the spore suspension is 0.5×10 6 individual / mL.

[0088] 3. Transfer the spore suspension prepared in step 2 to a PDA liquid medium for culture (25° C., 200 rpm, 48 h) at a 5% inoculum size to obtain a liquid culture.

[0089] 4. Centrifuge the liquid culture obtained in step 3 at 6000 rpm for 30 minutes to collect mycelium, wash it twice with sterile physiological saline, and dry...

Embodiment 2

[0115] Embodiment 2, the application of Acremonium acremonium FA1603

[0116] 1. Seed intensive cultivation of Acremonium acremonium FA1603

[0117] 1. Inoculate Acremonium acremonium FA1603 onto a PDA solid medium slant (18×180 mm test tube), and culture at 25° C. for 5 days.

[0118] 2. Transfer the bacterial strain cultivated in step 1 to a PDA solid medium slant (18×180 mm test tube) containing 20 μg / mL sodium selenite, and culture at 25° C. for 3 days.

[0119] 3. The spores on the PDA solid medium slant obtained in step 2 were eluted with 20mL sterile physiological saline (the spore concentration in the eluate obtained was 0.5×10 5 individual / mL), all the eluate was transferred to an eggplant bottle containing 100 g seed strengthening medium containing 50 μg / mL sodium selenite, and cultivated in an incubator at 25 ° C for 3 days to obtain a strengthened seed culture ( The culture spore concentration can reach 1012 per gram of culture).

[0120] 2. Acremonium acremoniu...

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Abstract

The invention discloses a selenium-enriched Acremonium acremonium strain and application thereof. Acremonium acremonium FA1603 provided by the present invention has a preservation number in China Center for Type Culture Collection as CCTCC NO: M2016331. The invention also protects a acremonium acremonium culture and a method for cultivating acremonium acremonium FA1603. Acremonium acremonium FA1603 is fermented to produce selenium-enriched acremonium acremonium with high content of cordycepin. The culture product of acremonium acremonium has a high content of organic selenium, especially active selenomethionine, and is rich in active ingredients of Cordyceps, especially the content of cordycepin. For feed addition, it is safe, non-toxic and has no side effects. It can significantly improve the nutrition of animal meat, improve the quality of meat, and at the same time promote the healthy development of animals and improve the immunity of the body, which has great economic and social benefits.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a selenium-enriched Acremonium acremonium strain and application thereof. Background technique [0002] Traditionally, Cordyceps is a general term for all fungi of the genus Cordyceps sensu lato in the ergot family Clavicipitaceae, and is the fungus that forms "Cordyceps". At present, there are more than 500 names related to Cordyceps fungus reported and recorded in the world, and about 120 species have been reported in China. Cordyceps has a wide range of hosts, which can be parasitic animals (including insects, spiders, acarids and nematodes, etc.) or other fungi (such as fungi of the genus Elaphomyces Nees and the genus Claviceps Tul.). The complexes of some Cordyceps fungi and hosts can be directly used in traditional Chinese medicine, such as Cordyceps sinensis, Cordyceps militaris and cicadae, etc., and most of the Cordyceps fungi can also produce a variety of biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K10/18A23L31/00C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 黄钦耿黄建忠蒋顺进方文棋吴松刚
Owner FUJIAN NORMAL UNIV
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