Method for extracting colza oil and protein by using solid state fermentation aqueous enzymic method

A technology of solid-state fermentation and rapeseed oil, which is applied in the field of vegetable oil and protein extraction and processing, can solve the problems of toxin and anti-nutrient content and low extraction rate, and achieve low anti-nutrient content, low cost, and simple process equipment Effect

Inactive Publication Date: 2012-12-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the research on extraction of rapeseed oil and rapeseed protein by solid-state fermentation combined with aqueous enzymatic method has not been reported, and the existing methods for extracting oil and protein in rapeseed by aqueous enzymatic method have low extraction rate, high content of toxins and anti-nutritional substances in the product, etc. question

Method used

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  • Method for extracting colza oil and protein by using solid state fermentation aqueous enzymic method
  • Method for extracting colza oil and protein by using solid state fermentation aqueous enzymic method
  • Method for extracting colza oil and protein by using solid state fermentation aqueous enzymic method

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: Screening test for optimal parameters of rapeseed solid-state fermentation

[0033] 1 Materials and methods

[0034] 1.1 Materials and reagents

[0035] rapeseed commercially available protex-6L alkaline endoprotease Danish novo company

[0036] 1.2 Main instruments and equipment

[0037] pHS-25 acidity meter Shanghai Weiye Instrument Factory electronic analytical balance Merlot-Toledo Instruments (Shanghai) Co., Ltd. centrifuge Beijing Medical Centrifuge Factory Precision electric mixer Ronghua Instrument Manufacturing Co., Ltd., Jintan City, Jiangsu Province Electric constant temperature water bath Yuyao Dongfang Electrical Instrument Factory Semi-automatic azotometer Shanghai Xinjia Electronics Co., Ltd. Digester Shanghai Fiber Inspection Instrument Co., Ltd. F2102 plant sample pulverizer Tianjin Test Instrument Co., Ltd. Soxhlet extractor Tianjin Glass Instrument F...

Embodiment 2

[0073] Embodiment 2: the screening test of the optimal parameter of rapeseed enzymatic hydrolysis

[0074] Based on the best solid-state fermentation process for extracting rapeseed oil and rapeseed protein determined in Example 1, a single-factor rapeseed enzymatic hydrolysis test was performed to determine the range of parameters for the rapeseed enzymatic hydrolysis process. Taking the oil extraction rate and protein extraction rate as the indicators, a response surface design experiment with 5 factors and 5 levels was carried out.

[0075] 1 Materials and methods

[0076] 1.1 Materials and reagents

[0077] rapeseed commercially available protex-6L alkaline endoprotease Danish novo company

[0078] 1.2 Main instruments and equipment

[0079] pHS-25 acidity meter Shanghai Weiye Instrument Factory electronic analytical balance Merlot-Toledo Instruments (Shanghai) Co., Ltd. centrifuge Beijing Medical Centrifuge Factory ...

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Abstract

A method for extracting colza oil and protein by using a solid state fermentation aqueous enzymic method belongs to an extracting process technology of vegetable fat and the protein. The method mainly comprises the following steps of: (1) adjusting moisture after smashing vegetable seeds to manufacture a fermentation medium, then conducting autoclaved sterilization, enabling activated mixed bacteria after cooling to inoculate to sterilized fermentation medium to conduct solid state fermentation to obtain fermentation products in certain proportion; and (2) stoving and smashing the fermentation products, mixing the smashed fermentation products with water to obtain a mixed liquor, adjusting a pH value and temperature of the mixed liquor, adding a protein enzyme in the mixed liquor to conduct enzymolysis, and conducting centrifugal separation after the enzymolysis to obtain the colza oil and protein. The method is simple in needed equipment, safe in operation, low in cost and high in extraction efficiency of the colza oil and the protein. Obtained colza oil is free of solvent vestigitals and high in nutritive value, and obtained colza protein is good in colza protein quality and low in anti-nutrition matter contents such as thioglycoside, phytic acid and tannin in products.

Description

technical field [0001] The invention belongs to the extraction and processing technology of vegetable oil and protein, in particular to a method for extracting rapeseed oil and protein by solid-state fermentation water enzymatic method. Background technique [0002] The enzymes secreted by microbial solid-state fermentation can effectively degrade or passivate anti-nutrients such as glucosinolates, phytic acid, and tannins in rapeseed. The aqueous enzymatic extraction technology is the most ideal method to obtain oil and protein at the same time from oil plants, so solid-state fermentation is used Combined water enzymatic method is a method to extract high-quality rapeseed oil and rapeseed protein. At home and abroad, some people have studied the extraction of oil and protein in rapeseed by aqueous enzymatic method. Jensen and Olsen (1990) used the complex enzyme of Aspergillus niger to grind the rapeseed twice, adjusted the pH value to 4.5, and kept it warm after adding enz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C12P21/06
Inventor 李杨江连洲齐宝坤于鹏曹亮
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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