Treatment of keratinous fibers with an enzyme having perhydrolase activity

A technology of perhydrolase and keratin fiber, which is applied in the direction of hydrolase, enzyme/microbe biochemical treatment, shrink-proof fiber, etc., and can solve the problems of harmfulness to textile workers

Inactive Publication Date: 2012-12-26
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chlorine-containing compounds and their decomposition products are harmful to the environment and textile workers

Method used

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  • Treatment of keratinous fibers with an enzyme having perhydrolase activity
  • Treatment of keratinous fibers with an enzyme having perhydrolase activity
  • Treatment of keratinous fibers with an enzyme having perhydrolase activity

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0127] Example 1: Morphological changes of wool fibers treated with enzymes

[0128] 50 mg of 100% wool fiber (ie, "top wool fiber") was incubated in a glass tube in a reaction volume of 2 ml at 65°C with slow agitation for one hour. The incubation conditions were as follows:

[0129] 1) buffer (100mM sodium phosphate buffer, pH 7),

[0130] 2) Buffer+5μl / ml PGDA+5μl / ml H 2 o 2 (50% w / w), or

[0131] 3) Buffer+5μl / ml PGDA+5μl / ml H 2 o 2 (50% w / w) + 0.8 ppm perhydrolase.

[0132] The particular perhydrolase used was the S54V variant of M. smegmatis perhydrolase (also known as "arylesterase"). After treatment, the wool fibers were rinsed three times with DI water and then air dried. The morphology of the treated wool fibers was evaluated using FEI's Phenom Scanning Electron Microscopy.

[0133] Use buffer+PGDA+H 2 o 2 processing wool fibers ( figure 2 ) compared to buffer-only treatment ( figure 1 ), did not significantly change the morphology of wool scales. Ho...

example 2

[0136] Example 2: Changes in dyeing affinity of enzymatically treated wool fibers

[0137] 50 mg of treated wool fibers (see Example 1) were dyed with Differential Textile Fiber Stain A (a combination of picric acid, G150 chlorazole blue and 3BS crocus scarlet) for 5 minutes at room temperature. A significant increase in dyeing affinity with Discriminative Fabric Fiber Stain A was observed in hydrolytic enzyme-treated wool fibers ( Figure 5 ). In use buffer or buffer+PGDA+H 2 o 2 While the treated wool fiber is dyed medium orange, with buffer or buffer + PGDA + H 2 o 2 + Perhydrolase-treated wool fibers are dyed deep red. These results demonstrate that treatment of wool fibers with perhydrolase increases dye uptake.

example 3

[0138] Example 3: Treated Wool Knit in Launder-O-meter reduced shrinkage

[0139] Three samples of 100% wool jersey (measured at 5" x 5"; test fabric type 532) were each placed in a washfastness tester at 65°C and one of the following conditions (n=3) Incubate for 1 hour with a reaction volume of 300ml (bath ratio = 32:1):

[0140] 1) buffer (100mM sodium phosphate buffer, pH 7),

[0141] 2) Buffer+5μl / ml PGDA+5μl / ml H 2 o 2 (50% w / w), or

[0142] 3) Buffer+5μl / ml PGDA+5μl / ml H 2 o 2 (50% w / w) + 0.8 ppm perhydrolase.

[0143] Representative results are in Image 6 shown in . Samples treated with perhydrolase showed greater 2 o 2 Treated samples showed significantly less shrinkage. These results demonstrate that treatment of wool fibers with perhydrolase reduces shrinkage under laundering conditions.

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Abstract

Described are compositions and methods relating to the treatment of keratinous fibers and textiles comprising such fibers with enzymatically-generated peracids in aqueous media. The treatment has beneficial effects, including reducing felting, increasing dye uptake, and reducing prickling tendency.

Description

technical field [0001] The compositions and methods of the present invention involve treating keratinous fibers and fabrics containing such fibers with enzymatically generated peracids under aqueous conditions. The treatment reduces felting and increases dye uptake. Background technique [0002] When textiles made of keratinous fibers, such as wool, are subjected to mechanical manipulation in a wet state, they have a tendency (sometimes dramatically) to shrink. This type of shrinkage is known as "felting". Felting is generally undesirable and irreversible, and renders the fabric or garment unusable. Felting tendencies can be mitigated by chemically modifying the surface of the textile which alters the frictional properties of the textile by reducing or masking the scale structure. [0003] Chlorination is the oldest and best known method used to reduce felting and increase dye uptake. However, chlorine-containing compounds and their decomposition products are harmful to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M11/50D06M11/82D06M16/00D06M11/55C12N9/14D06P5/22C12N9/10D06M101/12
CPCD06M11/50D06P5/22D06M16/006D06M11/55D06M11/82C12N9/1029D06M2200/45D06M16/003D06M2101/12
Inventor M-Y·允
Owner DANISCO US INC
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