Method and corresponding device for capturing and controlling in-vivo cells of living body animal

Abstract

The invention discloses a method for capturing and controlling in-vivo cells of a living body animal. The method comprises the following steps of: forming optical tweezers with a beam of laser, wherein the optical tweezers are an optical field which can be subjected to momentum exchange with particles; placing a part, which is required to be subjected to cell capturing, of the living body animal in the position where the optical tweezers are formed; aligning an illumination light beam with the optical tweezers and the part, which is required to be subjected to cell capturing, of the living body animal; and adjusting the relative position of the optical tweezers and the living body animal, so that the in-vivo cells of the living body animal are captured by the optical tweezers. According to the method, the in-vivo cells of the living body animal can be captured and controlled in a non-contact mode without damage to a dermic layer of the surface of the animal, and mechanical harm to the cells is avoided.

Description

technical field [0001] The invention belongs to the technical field of optical micro-manipulation, and in particular relates to a method for capturing and manipulating cells in a living animal and a corresponding device. Background technique [0002] Due to the complexity of the internal environment of animals, zoological research in vitro or in a culture environment may not accurately reflect the zoological activities in vivo, so in vivo testing of live animals is the ultimate verification of biological research. Studying biological processes such as the growth and metastasis of tumor cells and the interaction between cells and proteins in living animals is of great significance to the fields of life sciences, medical research and drug development. [0003] With the emergence and development of in vivo imaging techniques such as nuclear magnetic resonance, photoacoustic imaging, fluorescence, and bioluminescent imaging, the study of life activities in living animals has bec...

AI Technical Summary

Problems solved by technology

[0005] Atomic force microscope and micro suction technology belong to mechanical manipulation, magnetic tweezers can only manipulate magnetic microspheres, not biological cells

Therefore, none of these three techniques are suitable for manipulating cells in living animals

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