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Process for extracting polysaccharide from milkvetch roots

A technology of astragalus and polysaccharide, which is applied in the field of medicine, can solve the problems of decreased pharmacological activity of astragalus polysaccharide, influence of product purity and color, hydrolysis and destruction of astragalus polysaccharide, etc., and achieves the improvement of activity and immunity, high output rate, and maintenance of color and fragrance. Effect

Inactive Publication Date: 2013-01-16
NANTONG SIHAI PLANT EXTRACTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The polysaccharide content in natural Astragalus membranaceus is about 20%. At present, the extraction of Astragalus polysaccharides mainly includes water extraction and alcohol extraction. However, there are many problems in this extraction method: prolonged boiling and heating will cause Astragalus polysaccharides to be hydrolyzed and destroyed, resulting in The pharmacological activity of Astragalus polysaccharide decreases, and other components in Astragalus will be extracted at the same time, which has a great impact on the purity and color of the product
In addition, the extraction method in this way has a large workload, high cost, low yield and content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A process for extracting polysaccharides from Astragalus, comprising the steps of:

[0017] a, adopt 50g dry powder of Radix Astragali as raw material, control the particle diameter of dry powder of Radix Astragali to be 40 orders, dry powder of Radix Astragali is put into reaction vessel, then add 250g mixed solvent in reaction vessel, described mixed solvent is ethanol, water and n-butanol Mixture, wherein the weight ratio of ethanol, water and n-butanol=4:3:3;

[0018] b. Heat the solution after the mixed reaction in step a, control the heating temperature to 80°C, then add 6 g of multi-enzyme complex to the mixed solution, and enzymatically hydrolyze it for 1.5 hours. The multi-enzyme complex includes cellulose with a weight ratio of 1:1 Enzymes and pectinases;

[0019] c. Cool the mixed solution after the enzymolysis reaction, and control the temperature to 10° C., discard the lower precipitate after cooling, and obtain 150 g of supernatant;

[0020] d. Concentr...

Embodiment 2

[0023] A process for extracting polysaccharides from Astragalus, comprising the steps of:

[0024] a, adopt 100g Astragalus dry powder as raw material, control the particle diameter of Astragalus dry powder to be 50 orders, put Astragalus dry powder into reaction vessel, then add 500g mixed solvent in reaction vessel, described mixed solvent is ethanol, water and n-butanol Mixture, wherein the weight ratio of ethanol, water and n-butanol=4:3:3;

[0025] b. Heat the solution after the mixed reaction in step a, control the heating temperature to 85°C, then add 12 g of multi-enzyme complex to the mixed solution, and perform enzymatic hydrolysis for 2 hours. The multi-enzyme complex includes cellulase in a weight ratio of 1:1 and pectinase;

[0026] c. Cool the mixed solution after the enzymolysis reaction, and control the temperature to 15° C. After cooling, discard the lower precipitate to obtain 300 g of supernatant;

[0027] d. Concentrate the 300g supernatant obtained in s...

Embodiment 3

[0030] A process for extracting polysaccharides from Astragalus, comprising the steps of:

[0031] a, adopt 150g Astragalus dry powder as raw material, control the particle diameter of Astragalus dry powder to be 60 orders, put Astragalus dry powder into reaction vessel, then add 750g mixed solvent in reaction vessel, described mixed solvent is ethanol, water and n-butanol Mixture, wherein the weight ratio of ethanol, water and n-butanol=4:3:3;

[0032] b. Heat the solution after the mixed reaction in step a, control the heating temperature to 85°C, then add 18 g of multi-enzyme complex to the mixed solution, and perform enzymatic hydrolysis for 3 hours. The multi-enzyme complex includes cellulase in a weight ratio of 1:1 and pectinase;

[0033] c. Cool the mixed solution after the enzymolysis reaction, control the temperature to 20°C, discard the lower precipitate after cooling, and obtain 450g of supernatant;

[0034] d. Concentrate 450g of the supernatant obtained in ste...

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Abstract

The invention discloses a process for extracting polysaccharide from milkvetch roots. The process is characterized by including the following steps of step a, using a dry milkvetch root powder as a raw material, placing the dry milkvetch root powder in a reaction vessel, and adding a mixed solvent to the reaction vessel; step b, heating a solution obtained after the mixing reaction in the step a, and adding a multienzyme complex to the mixed solution, wherein the multienzyme complex comprises cellulose and pectinase with the weight ratio of 1:1; step c, cooling the mixed solution obtained after enzymolysis reaction, and removing lower layer precipitates after the cooling to obtain supernatant; step d, concentrating the supernatant obtained from the step c to be paste, adding ethanol for precipitation, and performing filtering to obtain a milkvetch root polysaccharide crude product; and step e, refining the milkvetch root polysaccharide crude product to obtain a milkvetch root polysaccharide refined product. The process for extracting the polysaccharide from the milkvetch roots has the advantages of being simple in operation, conditions are easy to achieve, the yield rate of the polysaccharide is high, and the purity is high.

Description

technical field [0001] The invention relates to a process for extracting polysaccharides from Radix Astragali, belonging to the technical field of medicine. Background technique [0002] Astragalus mainly contains polysaccharides, saponins, flavonoids, protein, cellulose and trace elements. The polysaccharide content in natural Astragalus membranaceus is about 20%. At present, the extraction of Astragalus polysaccharides mainly includes water extraction and alcohol extraction. However, there are many problems in this extraction method: prolonged boiling and heating will cause Astragalus polysaccharides to be hydrolyzed and destroyed, resulting in The pharmacological activity of astragalus polysaccharide decreases, and other components in astragalus will be extracted at the same time, which has a great impact on the purity and color of the product. In addition, this extraction method has a large workload, high cost, and low yield and content. Therefore, a new technical solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 倪继达
Owner NANTONG SIHAI PLANT EXTRACTS
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