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Telomerase activity detection kit and detection method thereof

A technology for activity detection and telomerase, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of limited application, difficulty in obtaining satisfactory sensitivity and detection range, etc. Sensitivity and detection range, the effect of high amplification efficiency

Inactive Publication Date: 2014-01-29
XI AN JIAOTONG UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Although these methods are simpler and faster, it is difficult to obtain satisfactory sensitivity and detection range. At the same time, some expensive or difficult-to-synthesize materials also limit the practical application of this type of method.

Method used

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  • Telomerase activity detection kit and detection method thereof
  • Telomerase activity detection kit and detection method thereof
  • Telomerase activity detection kit and detection method thereof

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Embodiment Construction

[0076] The present invention will be further described in detail below in conjunction with specific drawings and specific embodiments, which are explanations rather than limitations of the present invention.

[0077] see figure 1 , Schematic diagram of detection of telomerase activity based on ternary junction structure and base stacking-mediated isothermal cascade signal amplification, where a) is the reaction process of telomerase elongating substrate, b) and c) are based on DNA molecular machines Isothermal cascade signal amplification process.

[0078] Specifically, six oligonucleotide probes were used, including telomerase substrate, 3WJ template, 3WJ primer, primer1, primer2, and molecular beacon probe; their functions are stated as follows:

[0079] A telomerase substrate, which can be recognized by telomerase and undergo an elongation reaction; after being combined with telomerase, the reverse transcription activity of telomerase is used to extend the telomerase subst...

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Abstract

The present invention discloses a telomerase activity detection kit and a detection method thereof. According to the kit, a ternary linking structure probe is adopted to recognize a telomerase product, and a ternary linking structure triggered DNA molecule machine and a base stacking triggered DNA molecule machine are combined to achieve isothermal cascade nucleic acid amplification to convert a reverse transcription event of the telomerase in a tumor cell extract into a significantly-enlarged fluorescence detection signal so as to precisely determine telomerase activity in a trace amount of tumor cells and achieve detection on tumor cell telomerase activity inhibition by chemical drugs.

Description

technical field [0001] The invention belongs to the technical field of telomerase activity detection, and relates to a telomerase activity detection kit and a detection method thereof. Background technique [0002] Telomerase is a nucleoprotein reverse transcriptase consisting of an RNA template and a protein catalytic subunit. Through reverse transcription, it can extend the repeat sequence (TTAGGG)n at the 3′ end of the telomere of the chromosome, thereby stabilizing the length of the telomere and inhibiting cell division. In normal human tissues and cells, the activity of telomerase is inhibited, while in more than 85% of known tumor cells, telomerase often has higher activity, which is considered to be related to the activation, transformation and proliferation of tumor cells closely related. In the field of clinical diagnosis, telomerase is an important marker of malignant tumors. In addition, many cancer treatment methods and drugs also aim at inhibiting telomerase ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/48
Inventor 赵永席齐林陈锋赵越张青
Owner XI AN JIAOTONG UNIV