Method for collecting embryo from paeonia suffruticosa seed and for inoculation

A technology of peony seeds and tweezers, which is applied in the field of plant biology, can solve the problems of time-consuming, easily damaged embryos, easily damaged embryos, etc., and achieve the effects of reducing pollution rate, reducing waste, and avoiding damage

Inactive Publication Date: 2013-02-20
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This method directly cuts off half of the seeds, which is easy to damage the embryo, and the experimenter is bound to a certain extent when working in the ultra-clean workbench. demanding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A. Take 600 peony seeds, every 150 as a group;

[0021] B. Wash off the mucus on the peony seed surface with tap water;

[0022] C. Fix the peony seeds with your fingers, start from the back corresponding to the ventral suture of the peony seeds, start cutting from the end without the hypocotyl, cut to two-thirds of the height of the peony seeds, and keep the embryos in the remaining uncut In the peony seeds, the peony seeds that have been processed are washed with clear water at last, and set aside;

[0023] D. Wash the treated peony seeds again with water;

[0024] E. In the ultra-clean workbench, use tweezers to fix the two sides of the peony seed incision, break it apart, and keep the embryo on half of it, and take out the embryo with sterilized tweezers;

[0025] F. Put the radicle down and the cotyledon up, insert it vertically in the culture medium.

[0026] G. According to the statistics of the four groups of experimental results, 135, 137, 132, and 136 peony...

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PUM

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Abstract

The invention relates to a method for collecting embryos from paeonia suffruticosa seeds and for inoculation. The invention belongs to the plant biotechnology field. The method comprises the steps of material selecting, washing, paeonia suffruticosa seed cutting, sterilizing, embryo collecting, inoculating, and the like. Most of operations of the invention are completed outside an ultra-clean workbench, such that convenient operation of experiment operators is facilitated, working efficiency is improved, and pollution rate is greatly reduced. According to the invention, when a paeonia suffruticosa seed is cut, cutting is started on the back corresponding to a paeonia suffruticosa seed ventral suture, and is initiated from an end which does not contain a hypocotyls. Cutting is stopped at 2/3 of a paeonia suffruticosa seed height. Therefore, the possibility for embryo damage during seed cutting is greatly reduced, such that paeonia suffruticosa seed wasting is reduced.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for inoculating embryos from peony seeds. Background technique [0002] peony( Paeonia suffruticosa Andr.), elegant, magnificent, deeply loved by the people. With the improvement of people's living standards, the cultivation area of ​​peony in my country is increasing. The traditional breeding method has a long production cycle and high production cost, and at the same time, it is easy to cause the peony to be infected with viruses and the seed quality to decline. In order to realize the scale and industrialization of peony planting and reduce production costs, rapid propagation through tissue culture is an effective way. In the rapid propagation of peony tissue culture, the most used and most effective method is the mature embryo culture of peony. The first step in the cultivation of mature peony embryos is how to take out the embryos from the peony s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 施江郭倩辛莉董普辉史国安
Owner HENAN UNIV OF SCI & TECH
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