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Propolis flavonoid extracting method

An extraction method and a technology for propolis flavonoids, which are applied in pharmaceutical formulations, medical preparations containing active ingredients, unknown raw materials, etc., can solve problems such as incomplete flavonoid active ingredients, and achieve easy recovery, high application value, and energy consumption. less effect

Inactive Publication Date: 2013-02-20
广西蜜博士蜂业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for extracting propolis flavonoids, aiming to solve the problem of incomplete active ingredients of flavonoids extracted in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Mix 100g of propolis powder with 85% ethanol solution at a solid-to-liquid ratio of 1:15, soak for 20 hours at a temperature of 80°C; extract by reflux for 3 times, each time for 1 hour, and then combine the extracts; extract The liquid was concentrated under reduced pressure, and ethanol was recovered; the concentrated solution after removing ethanol was cooled to 5°C, and centrifuged for 20 minutes; the centrifuged supernatant was extracted twice with chloroform, and the obtained aqueous phase was extracted twice with ethyl acetate, and after extraction The obtained ethyl acetate extract was evaporated by a rotary evaporator to remove the ethyl acetate to obtain 4.08 g of flavonoids.

example 2

[0017] Mix 100g of propolis powder with 90% ethanol solution at a solid-to-liquid ratio of 1:20, soak for 18 hours at a temperature of 80°C; extract by reflux for 3 times, each time for 1 hour, and then combine the extracts; extract The liquid was concentrated under reduced pressure to recover ethanol; the concentrated solution after removing ethanol was cooled to 8°C and centrifuged for 30 minutes; the centrifuged supernatant was extracted twice with chloroform, and the obtained aqueous phase was extracted twice with ethyl acetate. The obtained ethyl acetate extract was distilled off by a rotary evaporator to obtain 4.03 g of flavonoids.

example 3

[0019] Mix 100g of propolis powder with 80% ethanol solution at a solid-to-liquid ratio of 1:20, soak for 24 hours at a temperature of 80°C; extract by reflux for 3 times, each time for 1 hour, and then combine the extracts; extract The liquid was concentrated under reduced pressure to recover ethanol; the concentrated solution after removing ethanol was cooled to 8°C and centrifuged for 30 minutes; the centrifuged supernatant was extracted twice with chloroform, and the obtained aqueous phase was extracted twice with ethyl acetate. The obtained ethyl acetate extract was distilled off by a rotary evaporator to obtain 4.12 g of flavonoids.

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Abstract

The invention provides a propolis flavonoid extracting method which is characterized by including steps of (1), mixing propolis with ethanol solution, soaking at the temperature of 70-85 DEG C for 16-24 hours; (2), refluxing and extracting the solution to obtain leaching liquid; (3) concentrating the leaching liquid to recover ethanol; (4) cooling and centrifuging condensed liquid without ethanol; and (5), extracting supernatant obtained by centrifuging twice by chloroform to obtain water phase, extracting the water phase twice by ehyl acetate, evaporating off the ehyl acetate from the ehyl acetate extract through a rotary evaporator so as to obtain flavonoid compounds. By the method, flavonoid effective components are complete, damage to active components is reduced, ethanol can be recovered easily, the process is simple, and time and energy consumption is low; further, the method is quite suitable for industrial production; and the prepared high-purity propolis flavonoid has high application value in medical healthcare, and has unique special function in cancer resistance.

Description

technical field [0001] The invention belongs to the field of compound extraction, in particular to a method for extracting propolis flavonoids. Background technique [0002] Propolis is a fragrant colloid that honey collects from new shoots or bark of plants, mixed with its own secretion. 40,000 to 50,000 bees can only collect about 40g-60g of propolis a year, known as "purple gold". Propolis Emperor is the best product in propolis, its total flavonoid content is as high as 9300mg, and the rest of the ingredients are in a golden ratio, synergistic. Scientists have given propolis many good names: vascular scavenger, blood sugar patron saint, anti-cancer warrior, natural immune enhancer. [0003] Existing scientific and technological means can separate more than 20 kinds of flavonoids from propolis, which is the main active substance in propolis and accounts for about 4.13% of propolis. These flavonoids include flavonoids, flavonols, and flavonoids. Flavonoids include chry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00A61K35/644
Inventor 周桂华
Owner 广西蜜博士蜂业有限责任公司
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