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Production of infectious bronchitis virus and vaccine from cell line

A chicken infectious and bronchitis technology, applied in the direction of antiviral agents, viruses/bacteriophages, microorganisms, etc., can solve problems such as interference, shortage of SPF chicken embryos, and artificial transmission of epidemic patients

Active Publication Date: 2013-03-13
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the vaccine has good safety and ideal immune effect, the production of chicken embryo seedlings requires a large number of SPF chicken embryos. The preparation method is not only labor-intensive and the output is low, but also faces the problem of the current shortage of SPF chicken embryos in my country.
The use of general chicken embryos to make seedlings may not only be interfered by maternal antibodies in chicken embryos, making the vaccine potency unstable, but also embryo-derived pathogens are very easy to cause artificial transmission of certain diseases along with the development, production, distribution and use of vaccines.

Method used

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  • Production of infectious bronchitis virus and vaccine from cell line
  • Production of infectious bronchitis virus and vaccine from cell line
  • Production of infectious bronchitis virus and vaccine from cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Vero cell production chicken infectious bronchitis virus and chicken infectious bronchitis inactivated vaccine

[0031] In this embodiment, Vero cells (African green monkey kidney cell line, purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) were selected as cell lines for producing chicken infectious bronchitis virus and chicken infectious bronchitis inactivated vaccine , Avian infectious bronchitis virus M41 strain virus seed was purchased from China Veterinary Drug Control Institute. The formula of the cell growth medium used is: 94% v / v DMEM (produced by Invitrogen Company) liquid, 6% v / v fetal calf serum (produced by PAA Company), the pH value is adjusted to 7.2; The formula of used cell maintenance liquid is: 99% v / v DMEM (manufactured by Invitrogen) and 1% v / v fetal bovine serum (manufactured by PAA) were adjusted to pH 7.2.

[0032] The following are the specific steps for using Vero c...

Embodiment 2

[0053] Embodiment 2 Vero cell production chicken infectious bronchitis virus and chicken infectious bronchitis live vaccine

[0054] In this embodiment, Vero cells (purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) were selected as the cell line for producing chicken infectious bronchitis virus and chicken infectious bronchitis live vaccine. The H120 strain of bronchitis virus was purchased from China Veterinary Drug Control Institute. The formula of the cell growth medium used is: 94% v / v DMEM (produced by Invitrogen Company) liquid, 6% v / v fetal calf serum (produced by PAA Company), the pH value is adjusted to 7.2; The formula of used cell maintenance liquid is: 99% v / v DMEM (manufactured by Invitrogen) and 1% v / v fetal bovine serum (manufactured by PAA) were adjusted to pH 7.2.

[0055] The following are the specific steps for using Vero cells to produce chicken infectious bronchitis virus and vaccine:

[...

Embodiment 3

[0071] Embodiment 3 Vero cell production chicken infectious bronchitis virus and chicken infectious bronchitis live vaccine

[0072]In this embodiment, Vero cells (purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) were selected as the cell line for producing chicken infectious bronchitis virus and chicken infectious bronchitis live vaccine. The H52 strain of bronchitis virus was purchased from China Veterinary Drug Control Institute. The formula of the cell growth medium used is: 94% v / v DMEM (produced by Invitrogen Company) liquid, 6% v / v fetal calf serum (produced by PAA Company), the pH value is adjusted to 7.2; The formula of used cell maintenance liquid is: 99% v / v DMEM (manufactured by Invitrogen) and 1% v / v fetal bovine serum (manufactured by PAA) were adjusted to pH 7.2.

[0073] The following are the specific steps for using Vero cells to produce chicken infectious bronchitis virus and vaccine:

[00...

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Abstract

The invention provides a method for production of an infectious bronchitis virus from a cell line. The employed cell line can be a Vero cell line, a BHK-21 cell line, a PK-15 cell line, a Marc145 cell line, a ST cell line, and an IBRS-2 cell line. The method includes: when the cell line forms a well-grown cell monolayer, inoculating an infectious bronchitis virus and making it adsorbed to the cell line; using a cell maintenance medium to conduct culture, adding an incubation agent to incubate the cell for 20-40min, and performing infectious bronchitis virus proliferation; and when CPE reaches over 75%, harvesting the infectious bronchitis virus. The method for production of an infectious bronchitis virus vaccine from a cell line provided in the invention can achieve the purpose of more secure and more effective infectious bronchitis virus and vaccine production.

Description

technical field [0001] The invention relates to a chicken infectious bronchitis virus produced by a cell line, a chicken infectious bronchitis live vaccine or an inactivated vaccine and a preparation method thereof, belonging to the field of animal vaccines. Background technique [0002] Avian Infectious Bronchitis (IB) is a highly contagious viral infectious disease caused by Avian Infectious Bronchitis Virus (IBV). IBV mainly causes respiratory disease and nephritis in chickens accompanied by a decline in egg production and egg quality. At present, the attenuated chicken embryo tissue vaccines of attenuated strains IB H120 and IB H52 and the inactivated chicken embryo tissue vaccines of IB M41 are mostly used for the prevention and treatment of this disease. Although the vaccine has good safety and ideal immune effect at present, the production of chicken embryo seedlings requires a large number of SPF chicken embryos. The preparation method is not only labor-intensive an...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/02A61K39/215A61P31/14
Inventor 张许科孙进忠
Owner PU LIKE BIO ENG
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