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Detection method of photochemical inactivation effect of methylene blue on HCV pathogen

A technology of chemical inactivation and detection method is applied in the field of PCR technology detection and evaluation of the effect of methylene blue light chemical method HCV virus inactivation, which can solve the problems of inactivation of the virus, difficult processing and high cost, and achieves short experimental period and avoids Infection, simple operation effect

Inactive Publication Date: 2013-03-13
SHANGHAI BLOOD CENT
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Problems solved by technology

The above method is difficult and expensive to deal with, and cannot be widely used in the virus inactivation of a single bag of plasma (such as 100 ml, 200 ml) for clinical use.

Method used

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  • Detection method of photochemical inactivation effect of methylene blue on HCV pathogen
  • Detection method of photochemical inactivation effect of methylene blue on HCV pathogen
  • Detection method of photochemical inactivation effect of methylene blue on HCV pathogen

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Embodiment Construction

[0024] The invention provides a method for detecting the inactivation effect of HCV virus by methylene blue light chemical method, using pseudovirus particles constructed by enveloped RNA technology as quality control products, and performing methylene blue light on blood and quality control products under the same conditions. For chemical inactivation, PCR detects the change in the amount of viral nucleic acid of the quality control before and after inactivation, and judges the virus inactivation effect. The inactivation effect of the quality control is the HCV virus inactivation effect. The structure of the HCV virus-like particle recombinant expression plasmid is as follows: Figure 4 shown.

[0025] The detection method of the present invention will be further described and introduced through specific examples below, so as to better understand the present invention, but the following examples do not limit the scope of the present invention.

[0026] 1. Construction of En...

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Abstract

The invention provides a method for detection and evaluation of photochemical virus inactivation effect of methylene blue, which adopts HCV pseudovirion constructed by enveloped RNA technology as a quality control, and performs methylene blue photochemical virus inactivation of the quality control and the blood or blood products needing to be subjected to virus inactivation under a same conition. The method has no virus infection risk to human body, and can reflect the disappearance condition of HCV infection by the damage degree of pseudovirion nucleic acid.

Description

technical field [0001] The invention relates to a method for detecting virus inactivation, in particular to a method for detecting and evaluating the effect of methylene blue photochemical method for inactivating HCV virus by PCR technology. Background technique [0002] Blood transfusion is a common medical measure for clinical treatment and rescue of patients. In recent years, with the continuous advancement of medical technology, the safety of blood and blood products has attracted more and more attention. Blood transfusion safety is not only the expected goal pursued by blood transfusion medicine and clinical therapeutics, but also a hot and extremely sensitive eternal topic of common concern among blood collection and supply institutions, medical institutions and even all walks of life. [0003] At present, it is known that a variety of viruses can be transmitted through blood, such as HIV1 / 2, HTLVI / II, hepatitis virus HBV and HCV, HDV and HEV, HAV, non-A~E hepatitis v...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 张博郑岚王迅黄宇闻莫琴钱开诚
Owner SHANGHAI BLOOD CENT
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