Chicken subunit triple vaccine, as well as preparation and application thereof

A technology of triple vaccine and vaccine adjuvant, which is applied in the direction of medical preparations containing active ingredients, antibody medical ingredients, antiviral agents, etc., which can solve the problem of interfering with laboratory diagnosis of virus isolation, easy to cause respiratory side effects, and immune susceptibility to maternal Antibody interference and other issues

Inactive Publication Date: 2013-03-20
QINGDAO VLAND BIOTECH INC +1
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Newcastle disease live vaccine is effective, cheap and easy to use, it has a short immunization period and is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, Newcastle disease inactivated vaccines have been used Vaccine has gradually become one of the routine methods for preventing and controlling Newcastle disease in chicken farms in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chicken subunit triple vaccine, as well as preparation and application thereof
  • Chicken subunit triple vaccine, as well as preparation and application thereof
  • Chicken subunit triple vaccine, as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] The main structural protein VP2 protein of IBDV is the most important host protective antigen. The present invention amplifies the super-virulent VP2 gene of IBD by PCR method, clones it on the pET-28a vector, transforms BL21(DE3), and induces it with IPTG After the VP2 protein is expressed, the expressing cells are homogenized under high pressure and then centrifuged to obtain the supernatant, which is the VP2 protein solution.

[0013] Newcastle disease virus Clone30 strain was inoculated into 10-day-old SPF embryos, and the chicken embryo liquid cultured for 48-96 hours was collected. The virus liquid was concentrated by ultrafiltration and concentration, and 0.1% formaldehyde was added to the final concentration.

[0014] Chicken infectious bronchitis virus M42 strain was used to inoculate 10-11-day-old SPF embryos, and the chicken embryo fluid cultured for 24-48 hours was collected. The virus was concentrated by ultrafiltration and concentration, and 0.1% formaldehy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention aims at providing a vaccine capable of simultaneously preventing infection of newcastle disease, infectious bronchitis and infectious bursal disease, in particular to very virulent strains. The vaccine comprises a infectious bursal disease VP2 protein with sequence of SEQ ID NO:1, newcastle disease and infectious bronchitis virus. The triple vaccine is used for immunizing a 21-day chick, which can simultaneously prevent newcastle disease, infectious bronchitis and infectious bursal disease, and has similar immune effect as newcastle disease single vaccine, infectious bronchitis inactivated vaccine and infectious bursal disease single vaccine, so that the aims of reducing the number of immunization times and reducing stress are achieved.

Description

technical field [0001] The invention belongs to the field of poultry vaccine manufacture, and in particular relates to a chicken subunit triple vaccine and its preparation and application, that is, a chicken Newcastle disease-infectious bronchitis-infectious bursal disease triple vaccine. Background technique [0002] Newcastle disease (ND) is an acute infectious disease of poultry caused by Newcastle disease virus (NDV). Although the Newcastle disease live vaccine is effective, cheap and easy to use, it has a short immunization period and is easily interfered by maternal antibodies, and it is easy to cause respiratory side effects and interfere with laboratory diagnosis of virus isolation. Therefore, since 1980, Newcastle disease inactivated vaccines have been used Vaccines have gradually become one of the conventional methods for preventing and controlling Newcastle disease in chicken farms in my country. [0003] Chicken infectious bursal disease (Infectious Bursal Diseas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/295A61P31/14A61K39/12A61K39/17
Inventor 王雷凌红丽蒋皓静杨光烈
Owner QINGDAO VLAND BIOTECH INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap