Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms

A technology of hyperin and hyperin from leaves of Acanthopanax senticosus, which is applied in the field of extraction and preparation of hyperin, can solve the problems of pollution, large investment, and large consumption of organic solvents, and reduce production costs, increase yield, The effect of easy operation

Active Publication Date: 2013-03-20
HENAN SOAR VETERINARY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the means of obtaining active ingredients are the application of classical extraction and separation methods, that is, organic solvents of different polarities are selected as extraction solvents to obtain components of different polarities, and the di

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] An enzyme-assisted extraction process for hyperin in Acanthopanax leaves, comprising the following steps:

[0023] (1), take Acanthopanax ( Acanthopanax Senticosus (Rupr.et Maxim.) Harms, bionic cultivar) leaf 40 mesh coarse powder 1000g, add distilled water of 2 times its mass, and then add a mixture of cellulase and pectinase with a mass ratio of 2:1 to make it (refer to Mixture, the same as below) reaches 0.2 mg / ml, adjust the pH to 4.5 with dilute hydrochloric acid with a mass concentration of 10%, heat to 50°C, enzymolyze for 2 hours, then heat up to 70°C to inactivate, and obtain the enzymolyzate;

[0024] (2) Add 90% ethanol 7 times its mass to the enzymolysis solution, soak for 1.5 hours, extract by percolation, collect the percolation solution until it is colorless, recover ethanol, and obtain a concentrated solution;

[0025] (3) Dilute the concentrated solution with distilled water twice its mass, and filter to obtain the supernatant;

[0026] (4) Apply HPD...

Embodiment 2

[0029] An enzyme-assisted extraction process for hyperin in Acanthopanax leaves, comprising the following steps:

[0030] (1), take Acanthopanax ( Acanthopanax Senticosus (Rupr.et Maxim.) Harms, bionic cultivar) 1000g of leaf 50-mesh coarse powder, add 2.5 times the amount of distilled water, and then add a mixture of cellulase and pectinase with a mass ratio of 2.5:1 to make it reach 0.25 mg / ml, dilute hydrochloric acid with a mass concentration of 8% to adjust the pH to 4.0, heat to 40°C, enzymatically hydrolyze for 2 hours, and then heat up to 75°C to inactivate to obtain an enzymolyzed solution;

[0031] (2) Add 90% ethanol 8 times its mass to the enzymolysis solution, soak for 2 hours, extract by percolation, collect the percolation solution until it is colorless, recover ethanol, and obtain a concentrated solution;

[0032] (3) Dilute the concentrated solution with distilled water 2.5 times its mass, and filter to obtain the supernatant;

[0033] (4) Apply HPD600 macro...

Embodiment 3

[0036] An enzyme-assisted extraction process for hyperin in Acanthopanax leaves, comprising the following steps:

[0037] (1), take Acanthopanax ( Acanthopanax Senticosus (Rupr.et Maxim.) Harms, bionic cultivar) 1000g of leaf 60-mesh coarse powder, add distilled water 3 times its mass, and then add a mixture of cellulase and pectinase with a mass ratio of 3:1 to make it reach 0.3 mg / ml, dilute hydrochloric acid with a mass concentration of 12% to adjust the pH to 5.0, heat to 60°C, enzymatically hydrolyze for 1.5h, and then heat up to 80°C to inactivate to obtain the enzymatic hydrolyzate;

[0038] (2) Add 95% ethanol of 6 times its mass to the enzymolysis solution, soak for 2 hours, extract by percolation, collect the percolation solution until it is colorless, recover ethanol, and obtain a concentrated solution;

[0039] (3) Dilute the concentrated solution with distilled water three times its mass, and filter to obtain the supernatant;

[0040] (4) Wash the supernatant wi...

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Abstract

The invention discloses an enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms. The technology comprises the following steps: taking crude powder of leaves of Acanthopanax senticosus Harms, adding water having a mass 1-2 times higher than the crude powder, adding a mixture of cellulase and pectase until the concentration of the mixture reaches 0.2-0.3mg/ml, adjusting the pH of the obtained solution to 4.0-5.0, heating to 40-60DEG C, carrying out enzymatic hydrolysis for 1.5-2h, heating to 70-80DEG C, and inactivating, wherein the mass ratio of the cellulase to the pectase of the mixture is (2-3):1; adding 90-95% ethanol, immersing, carrying out percolating extraction, collecting the obtained percolating liquid, and recovering ethanol to obtain a concentrate; diluting the concentrate by adding water having a mass 1-2 times higher than the concentrate, and filtering to obtain a supernatant; adding the supernatant into a macro-porous adsorption resin, eluting by water, eluting by 70-75% ethanol, collecting the obtained eluate, recovering ethanol, and carrying out reduced pressure drying to obtain concentrate powder; and carrying out reversed-phase resin column chromatography of the concentrate powder, eluting by 20-30% ethanol, collecting the obtained eluate, recovering ethanol to obtain crude crystals, and re-crystallizing methanol to obtain the hyperin. The technology, which adopts leaves of Acanthopanax senticosus Harms as a raw material, adopts the enzyme-assistant extraction method and adopts ethanol as a solvent to elute, has the advantages of environmental protection, no pollution, convenient operation and production cost reduction.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of hyperin, and in particular relates to an enzyme-assisted extraction process of hyperin in Acanthopanax leaves. Background technique [0002] Acanthopanax is the dry root and rhizome of Acanthopanax senticosus (Rupr.etMaxim.) Harms of the Araliaceae plant, and the stems and leaves can also be used for medicinal purposes. Mainly distributed in Northeast my country and North China, the resources are very rich. The chemical components of Acanthopanax mainly include: glycosides, lignin, flavonoids, polysaccharides, trace elements and amino acids. In Chinese medicine, Acanthopanax senticosus has been widely used as a medicine for a long time, and it is a typical strengthening medicine. Modern pharmacological studies have shown that Acanthopanax can enhance the body's non-specific defense capabilities. In addition to immune regulation, anti-tumor, anti-aging, anti-radiation, anti-...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
Inventor 李攀登梁军李坤鹏
Owner HENAN SOAR VETERINARY PHARMA
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