Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation
A microbial fermentation and biopeptide technology, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to completely remove anti-nutritional factors, inability to completely control bitterness and odor, and low protein utilization. , to achieve the effect of easy large-scale industrial production, low production cost and good taste
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Embodiment 1
[0048] see figure 1 , prepare the biologically active peptide of this embodiment according to the following method:
[0049] 1. Mix 500kg of high-protein soybean meal, 200kg of glutamic acid bacterial protein, and 350kg of dephenolized cottonseed protein through a 40-mesh sieve to make a fermentation substrate.
[0050] 2. The fermentation substrate is mixed with water according to the material-water ratio of 1:1.2, and the moisture content of the material is adjusted to about 50%. , Cook and sterilize for 30min under the condition of 0.11MPa.
[0051] 3. When the temperature of the sterilized fermentation substrate drops to 50°C, add 1 kg of papain (400,000 U / g) at a ratio of 1‰ of the weight of the fermentation substrate, and keep warm for 12 hours for hydrolysis.
[0052] 4. Aspergillus niger, Candida tropicalis, Aspergillus oryzae and Bacillus subtilis were subjected to enrichment culture and liquid fermentation according to the following methods:
[0053] (1) Aspergill...
Embodiment 2
[0078] see figure 1 , prepare the biologically active peptide of this embodiment according to the following method:
[0079] 1. Mix 600kg of high-protein soybean meal, 100kg of glutamic acid bacterial protein, and 300kg of dephenolized cottonseed protein through a 40-mesh sieve to make a fermentation substrate.
[0080] 2. The fermentation substrate is mixed with water according to the material-water ratio of 1:1.2, and the moisture content of the material is adjusted to about 50%. , Cook and sterilize for 30min under the condition of 0.11MPa.
[0081] 3. When the temperature of the sterilized fermentation substrate drops to 50°C, add 1 kg of papain (400,000 U / g) at a ratio of 1.5‰ of the weight of the fermentation substrate, and keep it warm for 12 hours for hydrolysis.
[0082]4. Aspergillus niger, Candida tropicalis, Aspergillus oryzae and Bacillus subtilis were respectively subjected to enrichment culture and liquid fermentation according to the method described in Examp...
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Abstract
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Application Information
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