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Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation

A microbial fermentation and biopeptide technology, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to completely remove anti-nutritional factors, inability to completely control bitterness and odor, and low protein utilization. , to achieve the effect of easy large-scale industrial production, low production cost and good taste

Active Publication Date: 2013-03-27
张有聪
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  • Description
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves how well we use yeast or other fungi to make various types of bioactive compounds from raw materials like nutrients found naturally within plants. By mixing these organism cells together during their growth cycle, they release different substances called oligopeptides which help them grow better than traditional methods without adding chemical agents at all. These techniques allow us to creatively generate new molecules containing specific functions while also simplifying manufacturing processes. Overall, this innovative approach allows companies to efficiently produce valuable bioactives based upon natural ingredients.

Problems solved by technology

Technologies aim at improving the quality and quantity of smaller peptidic substances (pepics) from natural resources such as plants or animals. These techniques involve separating them with various processes like extraction, ion exchange resin treatment, synthetic chemistry, biochemistry, synthesis, etc., which can lead to contamination issues and increase manufacturing time and expenses. To address these challenges, new technologies involving organismal metabolites were developed overall.

Method used

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  • Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation

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Embodiment 1

[0048] see figure 1 , prepare the biologically active peptide of this embodiment according to the following method:

[0049] 1. Mix 500kg of high-protein soybean meal, 200kg of glutamic acid bacterial protein, and 350kg of dephenolized cottonseed protein through a 40-mesh sieve to make a fermentation substrate.

[0050] 2. The fermentation substrate is mixed with water according to the material-water ratio of 1:1.2, and the moisture content of the material is adjusted to about 50%. , Cook and sterilize for 30min under the condition of 0.11MPa.

[0051] 3. When the temperature of the sterilized fermentation substrate drops to 50°C, add 1 kg of papain (400,000 U / g) at a ratio of 1‰ of the weight of the fermentation substrate, and keep warm for 12 hours for hydrolysis.

[0052] 4. Aspergillus niger, Candida tropicalis, Aspergillus oryzae and Bacillus subtilis were subjected to enrichment culture and liquid fermentation according to the following methods:

[0053] (1) Aspergill...

Embodiment 2

[0078] see figure 1 , prepare the biologically active peptide of this embodiment according to the following method:

[0079] 1. Mix 600kg of high-protein soybean meal, 100kg of glutamic acid bacterial protein, and 300kg of dephenolized cottonseed protein through a 40-mesh sieve to make a fermentation substrate.

[0080] 2. The fermentation substrate is mixed with water according to the material-water ratio of 1:1.2, and the moisture content of the material is adjusted to about 50%. , Cook and sterilize for 30min under the condition of 0.11MPa.

[0081] 3. When the temperature of the sterilized fermentation substrate drops to 50°C, add 1 kg of papain (400,000 U / g) at a ratio of 1.5‰ of the weight of the fermentation substrate, and keep it warm for 12 hours for hydrolysis.

[0082]4. Aspergillus niger, Candida tropicalis, Aspergillus oryzae and Bacillus subtilis were respectively subjected to enrichment culture and liquid fermentation according to the method described in Examp...

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Abstract

The invention relates to a method for producing a high-efficiency biological peptide through enzymolysis and microbe fermentation, which comprises the following steps of: smashing a fermentation substrate, uniformly mixing, then adding water for material blending, and then cooking and sterilizing; when temperature is reduced to 50-55 DEG C, adding a proteolytic enzyme for hydrolyzation; after the hydrolyzation is finished, when the temperature is reduced to 28-35 DEG C, adding a mixed bacterium solution to the hydrolyzed fermentation substrate, uniformity stirring, and carrying out aerobic fermentation under the condition of constant temperature; and carrying out low-temperature drying on the fermented materials, and then smashing to obtain a high-efficiency biological peptide finished product. The fermentation substrate comprises the following materials in parts by weight: 50-60 parts of high-protein bean pulp, 10-20 parts of glutamic acid bacterial protein and 30-40 parts of dephenolized cottonseed protein, and the components are smashed and screened through a 40-mesh screen and then uniformly mixed. The mixed bacterium solution is obtained by mixing the bacterium solutions of aspergillus niger, candida tropicalis, aspergillus oryzae and bacillus subtilis according to the proportion of 1:1:1:1, and the mixed bacterium solution is inoculated into the hydrolyzed fermentation substrate in 5%-10% by weight.

Description

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Claims

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Application Information

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Owner 张有聪
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