Method for identifying Va gene type of tobacco by detection of anther culture molecular markers

Abstract

The invention discloses a method for identifying Va gene type of tobacco by detection of anther culture molecular markers. The resistance of a common potato virus Y (PVY) resistant resource assumes recessive gene (Va) control, whether a single-strain Va gene is a heterozygote and a homozygote needs to be identified in flue-cured tobacco anti-PVY breeding; and since the current PAPD marker 012V3695 and SCAR marker PVYME1 of the Va gene type lack codominant DNA (Deoxyribose Nucleic Acid) markers or repulsion phase markers of Va, the heterozygote and the homozygote can not be screened directly by molecular markers. The method comprises the steps of collecting single-plant anthers of tobacco to be identified, culturing haploid seedlings by use of the anthers, detecting the marker separation condition of the haploid seedlings, and identifying whether the single-strain Va gene is heterozygote and homozygote according to the marker separation condition. Compared with a traditional test cross method, the method disclosed by the invention has the advantages of saving time and labor relatively.

Description

Technical field: [0001] The invention belongs to the technical field of crop breeding, in particular to a target genotype screening technology for tobacco quality traits and disease resistance transfer. Background technique: [0002] Tobacco potato virus Y disease, also known as vein spot disease, is an aphid-borne viral disease caused by potato virus Y (PVY). In order to breed disease-resistant varieties, a number of PVY-resistant germplasm resources have been identified at home and abroad, such as VAM, V.SCR, etc., and disease-resistant Burley varieties TN86, TN90 and flue-cured tobacco varieties NC55, NC102, etc. have been bred. Resistance to most sources of resistance manifests as recessive gene (va) control, which is caused by deletion of the gene for PVY susceptibility. And developed molecular markers (RAPD, SCAR) associated with the Va site (Noguchi S., Mol Gen Genet, 1999, 262:822-829; Julio et al., TheorAppl Genet. 2006, 112(2): 335- 346.). Flue-cured tobacco ger...

AI Technical Summary

Problems solved by technology

However, there is a lack of co-segregated molecular markers that can distinguish VaVa from Vava. When backcrossing the va locus, molecular marker-assisted selection cannot be directly used.

Usually, the testcross method is used to screen Vava single plants. Each generation of backcross needs to be tested and screened once, which is time-consuming and laborious.

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