Novel technology method for extracting convallamarin from wild convallaria

Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting convallamarin from wild convallaria in great khingan, in particular to a novel method for purifying by using ultrasonic extraction, ultrafiltration separation, macroporous resin adsorption and combining with column chromatography. The method has the advantages that 1, an ultrasonic extraction technique is adopted, so that effective components are dissolved out rapidly; the loss of active components is less; 2, a macroporous resin purification technique is adopted, so that purification effects of the effective components are good; the method facilitates industrial mass production; and 3, ultrafiltration is conducted at a room temperature, so that structural changes, loss and biological activity reduction of the effective components caused by a heating effect are avoided; and the yield of the effective components is increased. The method adopts the ultrasonic extraction and a macroporous resin coupling technique; alcohol serves as an extraction solvent; the ultrafiltration separation is conducted; then macroporous resin purification is conducted; the column chromatography is adopted for further purification; and the purity of convallamarin in convallaria is increased to 98.7%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting lily of the valley from wild lily of the valley in Daxing'an Mountains, in particular to a new method utilizing ultrasonic extraction, ultrafiltration separation, macroporous resin adsorption and column chromatography purification . Background technique: [0002] Lily of the valley, Liliaceae Lily of the valley, aliased as perfume flower, deer bell flower, grass jade bell, grass magnolia, small reed, mountain corn, born in the edge of the forest under the forest. Huzhong, Tahe, Jiagedaqi and other places in Daxingan Mountains have production. [0003] Plant form: perennial herb. Rhizome slender, with nodes, where numerous branched fibrous roots grow. Leaves 2, rarely 3, petiole 8-22 cm long, several membranous sheath leaves at base, blade oblong or ovate-lanceolate, 6-20 cm long, 2-8 cm wide, attenuate at base, Scapes derived from rhizomes, slig...

AI Technical Summary

Problems solved by technology

[0004] In China, the solvent method is generally used to extract picroside, which has the disadvantages of high operating temperature, low yield, and long time consumption. The difference of the present invention is that the ultrasonic extraction method is mainly used, and the macroporous resin is used to purify lilypicroside in the later stage. The product Not only high yield, but also high purity