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Stationary liquid

A technology of fixative solution and buffer solution, which is applied in the field of fixative solution, which can solve the problems of insufficient storage time and scrapping of ELISA kits, and achieve the effects of increasing storage time and stability, reducing economic losses, and reducing scrapping

Active Publication Date: 2013-04-17
杭州联科生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there has been a blocking step after the coating step, and drying after sealing; but at present, there is no fixative specially used for immobilizing protein activity between the blocking and drying steps, so that the storage time is not long enough, resulting in the failure of ELISA kits. The pre-coated ELISA plate expired and was scrapped, resulting in considerable economic losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Preparation steps of Human IL-6 pre-coated microtiter plate:

[0010] 1. Preparation of coating solution: Dilute the anti-Human IL-6 capture antibody to 2ug / ml with PBS buffer solution with a phosphate molar concentration of 0.01mol / L and pH=7.2 to obtain a coating solution.

[0011] 2. Coating: Add 100ul of coating solution to the enzyme-labeled well, and coat overnight at 4°C;

[0012] 3. Blocking: Discard the coating solution, add 1% BSA-PBST blocking solution 300ul / well, and block at room temperature for 4 hours;

[0013] 4. Preparation of fixative solution: Add sucrose and trehalose to PBS buffer solution with phosphate molar concentration of 0.01mol / L and pH=7.3, so that the mass fraction of sucrose is 30%, and the mass fraction of trehalose is 30%. Fixative.

[0014] 5. Fixation: Discard the blocking solution, add 300ul of the above fixing solution per well, and fix at room temperature for 0.5 hours;

[0015] 6. Drying: Discard the fixative and dry ...

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PUM

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Abstract

A stationary liquid belongs to the technical field of in vitro diagnostic reagents. The stationary liquid takes a PBS buffer solution as a solvent, and cane sugar and trehalose as solute, wherein as for the PBS buffer solution, the molarity of phosphate group is 0.01 mol / L, and the pH value is 7.2-7.4; and in the stationary liquid, the mass percent of the cane sugar is 5-30 percent, and the mass percent of the trehalose is 5-30 percent. Compared with the prior art, the stationary liquid has the advantages that the stationary liquid is used for fixing the activity of the protein capturing an antibody ELISA, so as to finally prepare an ELISA-precoated plate which can be preserved for a long time at the temperature of 4 DEG C; and the saving time and stability of an ELISA kit are increased, so as to reduce the scrapped rate of the ELIS kit due to the reduction and loss of the activity of the pre-coated plate; and accordingly, the economic loss is reduced.

Description

technical field [0001] The invention relates to a fixative solution, which belongs to the technical field of biological in vitro diagnostic reagents. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) has become an internationally recognized gold standard for protein quantification due to its high sensitivity, easy access to samples, and simple operation. One of the key technologies in the preparation of ELISA kits is the preparation of antibody solid-phase carrier microtiter plates. The conventional preparation method of the pre-coated ELISA plate is to incubate the coating solution in the enzyme-labeled well for a certain period of time, then discard the coating solution, store it at 4°C for 1 month, and store it at -20°C for 3 months; After adding desiccant and vacuuming, it can be stored at 4°C for about 1 year, but this cannot meet the needs of long-term storage of ELISA kits. ELISA pre-coated microtiter plates require longer protein activity reten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 袁志波
Owner 杭州联科生物技术股份有限公司