Lactobacillus casei and method for producing exopolysaccharide by same
A technology of Lactobacillus casei and extracellular polysaccharide, applied in the field of Lactobacillus casei and the production of exopolysaccharide
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Embodiment 1
[0058] (1) According to 14% skim milk powder, 6% glucose, and 2.6% casein peptone, an improved skim milk powder culture solution was prepared and sterilized.
[0059] (2) Inoculate the Lactobacillus casei activation liquid into the culture liquid with an inoculation amount of 4% by volume, and ferment at 32°C for 12 hours to obtain a fermentation liquid. After the fermentation broth was heated in a boiling water bath for 15 minutes, it was centrifuged at 10,000 g for 20 minutes at 4°C, and the supernatant was collected. Add trichloroacetic acid with a mass concentration of 800 g / L to the supernatant to a final mass concentration of 40 g / L, place at 4°C for 12 h, centrifuge at 10,000 g for 20 min, collect the supernatant, and concentrate by rotary evaporation with a rotary evaporator. Add ethanol with a volume fraction of 95% to the concentrated supernatant to a final volume fraction of 75%, let it stand at 4°C for 12 hours, then centrifuge at 10,000g at 4°C for 20 minutes, col...
Embodiment 2
[0062] (1) Prepare an improved skim milk powder culture solution with 10% skim milk powder, 8% glucose, and 2.4% casein peptone, and sterilize it.
[0063] (2) Inoculate the Lactobacillus casei activation liquid into the culture liquid with an inoculation amount of 5% by volume, and ferment at 30°C for 16 hours to obtain a fermentation liquid. After the fermentation broth was heated in a boiling water bath for 15 minutes, it was centrifuged at 10,000 g for 20 minutes at 4°C, and the supernatant was collected. Add trichloroacetic acid with a mass concentration of 800 g / L to the supernatant to a final mass concentration of 40 g / L, place at 4°C for 12 h, centrifuge at 10,000 g for 20 min, collect the supernatant, and concentrate by rotary evaporation with a rotary evaporator. Add ethanol with a volume fraction of 95% to the concentrated supernatant to a final volume fraction of 75%, let it stand at 4°C for 12 hours, then centrifuge at 10,000g at 4°C for 20 minutes, collect the pr...
Embodiment 3
[0066] (1) Prepare the improved skim milk powder culture solution according to 8% skim milk powder, 4% glucose, and 2.0% casein peptone, and sterilize it.
[0067] (2) Inoculate the Lactobacillus casei activation liquid into the culture liquid with an inoculation amount of 3% by volume, and ferment at 34°C for 18 hours to obtain a fermentation liquid. After the fermentation broth was heated in a boiling water bath for 15 minutes, it was centrifuged at 10,000 g for 20 minutes at 4°C, and the supernatant was collected. Add trichloroacetic acid with a mass concentration of 800 g / L to the supernatant to a final mass concentration of 40 g / L, place at 4°C for 12 h, centrifuge at 10,000 g for 20 min, collect the supernatant, and concentrate by rotary evaporation with a rotary evaporator. Add ethanol with a volume fraction of 95% to the concentrated supernatant to a final volume fraction of 75%, let it stand at 4°C for 12 hours, then centrifuge at 10,000g at 4°C for 20 minutes, collec...
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