A method for removing fishy smell by breaking the wall of spirulina with high efficiency and low loss of nutrients
A technology of spirulina and deodorization, applied in the direction of food science, etc., can solve the problems of time-consuming, etc., achieve the effect of reducing the loss ratio and improving the effect of deodorization
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Embodiment 1
[0036] Embodiment 1 lysozyme-papain double enzyme method
[0037] 1. Weigh a certain amount of spirulina dry powder, add pure water and stir continuously to form a spirulina solution with a concentration of 12%, add lysozyme and papain to the spirulina solution, the dosage is 0.54mg / ml and 0.5mg / ml respectively ml, stirred evenly and then enzymatically hydrolyzed. During the enzymolysis process, ensure that the pH value of the solution is between 6.0 and 7.0, the temperature is 40°C, and the time is 1h. After the enzymatic hydrolysis, the wall breaking rate of Spirulina observed under a microscope was 97.3%, and the sensory evaluation was carried out at the same time, with a score of 80.9.
[0038] 2. After adding 4mg / ml of β-cyclodextrin to the hydrolyzed solution of Spirulina obtained in step 1, stir at 40°C for 40 minutes, filter and dry the deodorizing solution of Spirulina after masking treatment to obtain Spirulina Algae deodorization dry powder.
[0039] 3. Prepare 1...
Embodiment 2
[0042] Similar to Example 1, its difference is that during lysozyme-papain enzymolysis, the concentration of spirulina solution is 9%, the consumption of lysozyme is 0.135mg / ml, the consumption of papain is 1.125mg / ml, the enzymolysis temperature The temperature is 35°C and the time is 3h. The amount of β-cyclodextrin used for β-cyclodextrin embedding was 5 mg / ml, the temperature was 35° C., and the stirring time was 45 minutes.
[0043] After the enzymatic hydrolysis, the wall breaking rate of Spirulina observed under a microscope was 97.4%, and the sensory evaluation score was 78.6 points. The solution prepared by β-cyclodextrin-embedded spirulina deodorization dry powder has basically no fishy smell, the color is light blue-green (close to light blue), and the solution has basically no precipitation. The sensory evaluation score is 91.3 points, and the protein retention The rate is 95.2%.
Embodiment 3
[0045] Similar to Example 1, its difference is that during lysozyme-papain enzymolysis, the concentration of spirulina solution is 15%, the consumption of lysozyme is 0.45mg / ml, the consumption of papain is 1.25mg / ml, the enzymolysis temperature The temperature is 30°C, the time is 2h, the dosage of β-cyclodextrin is 5mg / ml when β-cyclodextrin is embedded, the temperature is 30°C, and the stirring time is 50min.
[0046] After the enzymatic hydrolysis, the wall breaking rate of Spirulina observed under a microscope was 98.1%, and the sensory evaluation score was 76.9. The solution prepared by β-cyclodextrin-embedded spirulina deodorized dry powder has basically no fishy smell, the color is light blue-green (close to light green), the solution basically has no precipitation, the sensory evaluation score is 90.4 points, and the protein retention rate 95.7%.
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