Method for interfering plant endogenous CLE family polypeptide hormone and antagonist polypeptide for small molecule polypeptide hormone

Abstract

The invention relates to the field of genetic engineering and in particular relates to a method for interfering plant endogenous CLE family polypeptide hormone and antagonist polypeptide for small molecule polypeptide hormone. According to the method, effective antagonist polypeptides for small molecule polypeptide hormones of CLV3, CLE1, CLE8 and CLE27 are obtained through amino acid replacement and are expressed in plants through a transgenosis method, the specific polypeptides are subjected to functional interference, the downstream signal transduction is blocked, the polypeptides are further subjected to functional research, and the potential application values on model plant Arabidopsis and crops are mined.

Description

Technical field [0001] The invention relates to the field of genetic engineering, in particular to a method for interfering with plant endogenous CLE family polypeptide hormones and an antagonistic polypeptide of small molecule polypeptide hormones. Background technique [0002] Since the discovery of systemin, the first polypeptide hormone in higher plants in the early 1990s, more than 20 small-molecule polypeptide hormones from different plants including Arabidopsis, rice, soybeans, etc. have been reported to participate in immune defense responses. , Nodule formation, cell division, stem cell maintenance and differentiation, stoma formation, anther tapetum development, pollen-stigma recognition, pollen tube guidance and petal shedding, and many other important biological processes. From the current research results, small molecule peptide hormones mainly play a role in close-range signal transduction. They interact with the receptor kinases of the LRR-RLK family on the surface...

AI Technical Summary

Problems solved by technology

However, there are still many problems in the functional research of plant polypeptide hormones using the existing methods.

For example, 1: Since the genes encoding polypeptide hormones are usually small, current gene mutation methods such as EMS mutagenesis and T-DNA insertion methods are difficult to mutate them; 2: Since polypeptide hormones function as signal molecules in plants , the content is usually very low, and it is difficult to isolate and detect these polypeptide hormones from plants with current technical means; 3: Since the genes encoding plant polypeptide hormones usually belong to a certain gene family, the encoded precursor proteins contain conserved polypeptides The coding region has redundant functions. Even if one of the members is mutated, it is difficult to see that the plant is abnormal, so it is impossible to study its function

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