High-efficiency and energy-saving validamycin fermenting method

A technology of Jinggangmycin and fermentation method, which is applied in the field of microbial fermentation, can solve problems such as waste of dissolved oxygen, premature aging of hyphae, and destruction of cell components, and achieve the effects of reducing energy consumption and increasing production

Inactive Publication Date: 2013-05-08
ZHEJIANG TONGLU HUIFENG BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the ventilation rate is not adjusted, it may cause a waste of dissolved oxygen. Excessively high dissolved oxygen will produce singlet (O), superoxide (O 2 - ) or hydroxyl radicals (OH - ), destroys many cellular components, leading to premature senescence of the hyphae, which may affect the production of metabolites

Method used

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  • High-efficiency and energy-saving validamycin fermenting method
  • High-efficiency and energy-saving validamycin fermenting method
  • High-efficiency and energy-saving validamycin fermenting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Inoculate the strain of Streptomyces hygroscopicus Jinggang into the liquid fermentation medium, control the inoculum amount to 12%, and the fermentation temperature is 40°C; the liquid fermentation medium contains 120g of rice flour, 20g of peanut cake powder, NaCl2g, CaCO 3 1g . ,KH 2 PO 4 1g, the pH value is 7;

[0022] 2) At the initial stage of fermentation, the ventilation volume should be controlled at 3000m 3 / h, ferment from 30h to the end of fermentation, control the dissolved oxygen to 30%, and control the stirring speed to 120r / min during the fermentation process.

Embodiment 2

[0024] 1) Inoculate Streptomyces hygroscopicus var. Jinggang into the liquid fermentation medium, control the inoculation amount to 8%, and the fermentation temperature is 35°C; the liquid fermentation medium contains 70g of rice flour, 10g of peanut cake powder, 1g of NaCl, and CaCO 3 1g . ,KH 2 PO 4 1g, pH 6;

[0025] 2) At the initial stage of fermentation, the ventilation volume should be controlled at 2500m 3 / h, ferment 25h to the end of fermentation, control the dissolved oxygen to 20%, and control the stirring speed to 50r / min during the fermentation process.

Embodiment 3

[0027] 1) Inoculate Streptomyces hygroscopicus varietal Jinggang into the liquid fermentation medium, control the inoculation amount to 15%, and the fermentation temperature is 45°C; the liquid fermentation medium contains 150g of rice flour, 30g of peanut cake powder, NaCl2g, CaCO 3 2g . ,KH 2 PO 4 3g, the pH value is 8;

[0028] 2) At the initial stage of fermentation, the ventilation volume should be controlled at 3500m 3 / h, ferment for 35h to the end of fermentation, control the dissolved oxygen amount to 50%, and control the stirring speed to 150r / min during the fermentation process.

[0029] According to embodiment 1, get fermentor A, fermenter B to carry out following test according to the step described in embodiment 1.

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Abstract

The invention discloses a high-efficiency and energy-saving validamycin fermenting method, belonging to the field of microbial fermentation technology. The method comprises the following technological steps: 1) inoculating streptomyces hygroscopicus strains into liquid fermentation culture medium and controlling the inoculum size to be 8-15%, wherein the fermentation temperature is at 35-45 DEG C; and 2) controlling the ventilator capacity to be 2500-3500m<3>/h at early fermentation stage, fermenting for 25-35h, and then controlling the dissolved oxygen to be 20-50% until the fermentation is finished. According to the method, the dissolved oxygen rule can be discovered by measuring and analyzing the dissolved oxygen, and the concentration of the dissolved oxygen is controlled to be 20-50% after the fermentation culture is conducted for 25-35h, so that the energy consumption of an air compressor can be effectively reduced, the total air usage amount can be saved by about 25%, the electricity consumption can be saved by 10% of total electricity consumption each year, and the production volume of the validamycin can be improved by more than 4%.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a high-efficiency and energy-saving Jinggangmycin fermentation method. Background technique [0002] Jinggangmycin is an antibiotic produced by Streptomyces hygroscopicus var. Jinggang, which is mainly used to control rice sheath blight. The production of Jinggangmycin adopts liquid submerged fermentation. Dissolved oxygen is an important control indicator in the process of liquid cultivation. The solubility of oxygen in the fermentation liquid is very small, and it is necessary to keep aerating oxygen to ensure the oxygen concentration in the fermentation liquid. The method of controlling dissolved oxygen is as follows: Increase the stirring speed, adjust the air intake and exhaust volume, etc. [0003] The fermentation process of Jinggangmycin consumes a lot of energy. In the process of liquid culture, the metabolic intensity of bacteria is differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/46C12R1/55
CPCY02P20/10
Inventor 李忠乐峰松殷红福陈明兆叶全标胡颖亮吴闯赵晓春
Owner ZHEJIANG TONGLU HUIFENG BIOSCI
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