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Method for detecting quality of quercetin in yellow spot removing capsules

A quality detection method, the technology of quercetin, is applied in the quality detection of quercetin in traditional Chinese medicine capsule preparations, and in the field of quality detection of active ingredients in pharmaceutical preparations, and can solve problems such as uneven quality, rough quality control, and different content , to achieve the effect of scientific method, quality assurance, qualitative and quantitative accuracy

Inactive Publication Date: 2013-05-22
CHENGDU LIST PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] What is obtained here is actually the contents of the capsules. After analysis, we can know that the quality control in the whole production process is relatively simple and rough. It may be due to different production batches, or even the same batch will produce different contents. Identical products with uneven quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Identification of quercetin

[0055] Take an appropriate amount of the content of Ganhuang Quban Capsule and grind it into small pieces, weigh 3.0 g of the powder, add 25 ml of ethyl acetate, reflux for 30 minutes in a water bath, filter, evaporate the filtrate to dryness, add 2 ml of methanol to the residue to dissolve it, and use it as the test solution . Another appropriate amount of quercetin reference substance was taken, and a solution containing 0.5mg per 1ml was prepared with methanol as the reference substance solution. According to the thin-layer chromatography test, draw 3 μl of each of the above two solutions, point them on the same silica gel G thin-layer plate, use toluene-ethyl acetate-formic acid (5:2:1) as the developing solvent, develop, take out, and dry. Dry, spray with 3% aluminum trichloride ethanol solution, heat at 105 ℃ for 2-20 minutes, take out, and inspect under ultraviolet lamp (365 nm). In the chromatogram of the test substance...

Embodiment 2

[0056] Example 2 Determination of the content of quercetin

[0057] Use octadecylsilane bonded silica gel as filler; use methanol-0.4% phosphoric acid aqueous solution (46:54) as mobile phase; detection wavelength is 370nm; theoretical plate number should not be less than 2000 calculated by quercetin peak. Accurately weigh an appropriate amount of quercetin reference substance, and use 80% methanol aqueous solution to prepare a reference substance solution containing 0.04 mg per 1 ml. Take about 0.5g of the powder content of Ganhuang Quban Capsule, accurately weigh it, put it in a conical flask, add 50ml of 80% hydrochloric acid methanol solution (preparation method: take 3 parts of methanol and 1 part of hydrochloric acid solution and mix), accurately weigh After determination, reflux under water bath conditions for 1.0 hours, take out, let cool, accurately weigh again and use 80% methanol aqueous solution to make up the weight, shake well, filter, and use the filtrate as t...

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Abstract

The invention discloses a method for detecting the quality of quercetin in yellow spot removing capsules, which belongs to the technical field of medicine quality control. The method carries out identification and content detection through thin-layer chromatography and high efficiency liquid chromatography respectively by taking the quercetin as a comparison product and the contents of the yellow spot removing capsules as a tested product. The high efficiency liquid chromatography is performed under the conditions that octadecyl silane bonded silica gel is taken as a filler, a methanol-0.4% phosphoric acid water solution (46:54) is taken as a mobile phase, the detection wavelength is 370 nanometers, and the number of theoretical plates is not less than 2000 by being calculated according to the peak of the quercetin. The method disclosed by the invention can be used for carrying out the identification and the content detection on the quercetin by the thin-layer chromatography and the high efficiency liquid chromatography respectively, is more scientific, more advanced in detection means and more accurate in quantitative and quantitative detection, and can not only better and more comprehensively reflect the effective components of the yellow spot removing capsules, but also prevent the condition that other medicinal materials substitute for penthorum chinense pursh for production, thereby ensuring the quality, the safety and the effectiveness of the medicine.

Description

technical field [0001] The invention relates to a quality detection method of active ingredients in pharmaceutical preparations, in particular to a quality detection method of quercetin in traditional Chinese medicine capsule preparations for treating chloasma, and belongs to the technical field of pharmaceutical quality control. Background technique [0002] The traditional Chinese medicine capsule preparation for the treatment of chloasma specifically involved in the present invention is Quban Capsule, which is prepared by adding appropriate auxiliary materials after making a thick ointment from 835g of Huangcao and 300g of Salvia Miltiorrhiza. The specific preparation method and use have been recorded in In the invention patent with the publication number CN101700276, the preparation has the effects of reducing jaundice, removing blood stasis and removing freckles, and is intended to be used clinically for the treatment of female chloasma caused by stagnation of liver qi. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90G01N30/06G01N1/28
Inventor 张玲张熠赵力科
Owner CHENGDU LIST PHARMA
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