Method for preparing heparin sodium through ultrasound enzymolysis

Abstract

The invention relates to a heparin sodium technology, and particularly relates to the background art of a technology for extracting heparin sodium through complex-frequency combination ultrasound-enhanced two-enzymes. The invention aims at providing a preparation method for extracting the heparin sodium through a two-enzyme method. The preparation method comprises the following steps of: extracting the heparin sodium by using two-enzyme combination for enzyme catalysis, and preparing the heparin sodium by selecting the variety of enzymes, proportion of enzyme combination and strength of an ultrasonic field and controlling time and other working procedures. Compared with the prior art, the preparation method has the advantages that 1. the time of whole working procedure is reduced by approximately 33%; 2. the yield of the product is increased by 29.6%; and 3. the titer is stabilized to be above 120 (the titer of products adopting the domestic advanced technology is 90-'0).

Description

technical field [0001] The present invention relates to a technology of heparin sodium, in particular to the background technology of the technology of double-enzyme extraction of heparin sodium combined with multiple frequency combination ultrasound. Background technique [0002] At present, almost all production in our country uses pig small intestine as raw material, and several methods such as salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis are used in conventional reactors, among which salt hydrolysis is the most common application, and its production process is: raw material (intestinal mucosa), conventional reactor Inner brine extraction, filtration, resin adsorption, elution, ethanol precipitation, dehydration, drying, crude heparin sodium. [0003] Although the process of producing heparin sodium by the above method is relatively simple and easy to realize, the weak point of existence is that the process is delicate, because the crude product...

AI Technical Summary

Problems solved by technology

[0003] Although the process of producing heparin sodium by the above method is relatively simple and easy to realize, the weak point of existence is that the process is delicate, because the crude product heparin sodium is through repeated acid-base treatment, which not only reduces the yield of heparin sodium, but also reduces the yield of heparin sodium. The biological activity is also greatly affected, especially the potency is difficult to reach more than 90USPu / mg, and the time is longer