Bacillus subtilis, biological prevention and control preparation thereof and application of biological prevention and control preparation

A technology for Bacillus subtilis and biological control preparations, applied in the directions of biocides, biochemical equipment and methods, and chemicals for biological control, etc. problems, to achieve good application prospects, strong stress resistance, and the effect of improving crop yield

Active Publication Date: 2013-06-05
山东戴盟得生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The breeding cycle of disease-resistant varieties is long and the resistance is single. However, chemical control can easily cause pesticide residues and environmental...

Method used

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  • Bacillus subtilis, biological prevention and control preparation thereof and application of biological prevention and control preparation
  • Bacillus subtilis, biological prevention and control preparation thereof and application of biological prevention and control preparation
  • Bacillus subtilis, biological prevention and control preparation thereof and application of biological prevention and control preparation

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Embodiment 1

[0029] 1. Isolation and purification of Bacillus subtilis (B.subtilis) XLBS-01

[0030] The Bacillus subtilis (B.subtilis) XLBS-01 of the present invention is obtained from the separation of the soil by dilution plate method and plate streak method, and the separation method is as follows:

[0031] (1) Isolation of Bacillus: Soil samples were collected from plots planted with different types of crops in different regions of the country, and a 5-point sampling method was used to collect appropriate amounts of soil around and in the center of the plot within a depth of 10-20cm. Mix in equal amounts. Indicate the location, time and person of the collection. Weigh 1 g of soil sample into 100 mL of sterile water, place it in a shaker at 30 °C at 150 rpm for 10 min, then place it in a water bath at 60 °C for 30 min, take 100 μL of 10 -2 、10 -3 、10 -4 Spread the diluted solution on the LB medium plate, and apply three parallels for each gradient. After culturing at 30°C for 2 day...

Embodiment 2

[0047] 1. Bacillus fermentation process

[0048] LB liquid medium: the formula is tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0049] Solid material: rice husk: corn flour: soybean meal flour: bran=55:25:15:5;

[0050] Inorganic salt solution: 20% potassium dihydrogen phosphate, 5% magnesium sulfate, 10% ammonium sulfate, 65% light calcium carbonate;

[0051] The solid-to-liquid ratio is 98.7:0.3 (mass ratio).

[0052] Mass solid fermentation process of Bacillus subtilis (B.subtilis) strain XLBS-01:

[0053] ① Strain seed liquid culture: pick a small amount of Bacillus subtilis strain (B.subtilis) XLBS-01 from the slant of the test tube, transfer it to LB liquid medium, shake it at 30°C for 3-5 days, and this is the seed liquid.

[0054] ②Cultivation of solid-producing strains: inoculate 10% of the seed solution into solid medium (500mL Erlenmeyer flask), incubate at a constant temperature of 30°C for 3-5 days, and ...

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Abstract

The invention discloses bacillus subtilis, a biological prevention and control preparation thereof and an application of the biological prevention and control preparation, and belongs to the field of biological plant disease and pest prevention and control. The stain is bacillus subtilis XLBS-01, and has the preservation number of CGMCC No. 6692. Tests show that the bacillus subtilis disclosed by the invention has the characteristics of high growing speed, large sporulation quantity, wide action spectrum, strong stress resistance, capability of realizing rapid and large-quantity colonization at plant roots, and the like, and therefore, the bacillus subtilis has good application prospects. The biological prevention and control preparation prepared from the bacillus subtilis can not only effectively prevent and control plant soil-borne diseases, but also can effectively increase the crop output, thereby being a biological prevention and control preparation with vast application prospects. The microbial preparation can be used as a biopesticide or a biofertilizer for preventing and controlling various soil-borne plant diseases and also preventing and controlling plant leaf diseases, such as hot pepper anthracnose, cucumber target spot, grape downy mildew, rose powdery mildew and the like.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Bacillus subtilis capable of efficiently preventing and controlling plant diseases and its use, and a biological control agent prepared by using the Bacillus. Background technique [0002] Plant diseases seriously affect the growth and product quality of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The breeding cycle of disease-resistant varieties is long and the resistance is single. Chemical control can easily cause pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in reduced cont...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/125
Inventor 牛赡光朱卫东王清海张淑静段春华刘幸红
Owner 山东戴盟得生物科技有限公司
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