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Fermentation liquid of bacillus hirsutum and sesquiterpene and its application

A technology of Bastia pilosa and fermentation liquid, which is applied in the fields of application, botany equipment and methods, gardening, etc., can solve the problems of long cycle, lack of commercial feasibility of extracting active ingredients, unstable biological active substances, etc., and achieve blood sugar reduction Effect

Active Publication Date: 2015-12-02
JIANGSU ANHUI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the excessive utilization of the wild resources of golden ears, the resources have formed a great threat
The traditional artificial cultivation method has a long cycle, and it takes several months to form a generation of fruiting bodies
The long-term cultivation process results in the instability of fruiting body components, especially those bioactive substances, making extraction of active ingredients from fruiting bodies less commercially viable

Method used

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  • Fermentation liquid of bacillus hirsutum and sesquiterpene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of fermented liquid and sesquiterpene crude product

[0027] 1. Use the newly-configured potato medium slant (Ge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P367) to transfer the bacteria strain of Basilis pilosae, the culture temperature is 27 ° C, after the mycelium is covered with the slant, The slant strains were inserted into 250 mL Erlenmeyer flasks (84 flasks in total) filled with 60 mL of shaker flask culture medium, and cultured on a shaker at 22° C. and 90 rpm for 24 hours. Wherein the formula of shake flask culture medium is (unit is gram / liter): glucose 5, corn meal 5, peptone 1, KH 2 PO 4 1. MgSO 4 1. The initial pH value is 5.0.

[0028]2. Put 5000mL of the cultured strain above into a 75L seed tank with 45L of seed culture medium, the total amount of fermentation liquid in the seed tank is 50L; maintain the tank temperature at 22°C, tank pressure at 0.05MPa, and stirring speed at 90 ...

experiment example 2

[0032] Experimental Example 2: Preparation of Fermented Broth and Crude Sesquiterpene

[0033] 1. Use the newly-configured potato medium slant (Ge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P367) to transfer the bacteria strain of Basilis pilosae, culture temperature 25 ° C, after the mycelium is covered with the slant, The slant strains were inserted into 250 mL Erlenmeyer flasks (total 40 flasks) equipped with 90 mL of shaking flask culture medium, and cultured on a shaker at 25° C. and 120 rpm for 36 hours.

[0034] 2. Put 4000mL of the cultured strain above into a 100L seed tank with 50L of seed culture medium, the total amount of fermentation liquid in the seed tank is 54L; maintain the tank temperature at 25°C, the tank pressure at 0.05MPa, and the stirring speed at 120 rpm , ventilation rate 1:0.5v / v / m, culture time 90 hours.

[0035] 3. Insert the 30L seed tank bacteria into a 500L fermenter with 300L fermentation medium. In th...

Embodiment 3

[0042] Embodiment 3: the preparation of the fermented liquid of bacillus pilosa and the crude product of sesquiterpene

[0043] 1. Use the newly-configured potato medium slant (Ge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P367) to transfer the bacteria strain of Basilis pilosae, and the culture temperature is 30 ° C. After the mycelium is covered with the slant, The slant strains were inserted into 250mL Erlenmeyer flasks (10 flasks in total) equipped with 150mL shake flask culture medium, and cultured on a shaker at 30°C and 150 rpm for 46 hours.

[0044] 2. Put 3000mL of the strain cultured in this liquid shake flask into a 30L seed tank equipped with 50L first-level seed medium, and the total fermented liquid in the seed tank is 31.5L; maintain the tank temperature at 30°C, and the tank pressure at 0.05MPa, The stirring speed is 150 rpm, the ventilation rate is 1:1 v / v / m, and the fermentation time is 90 hours.

[0045] 3. Insert 15...

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Abstract

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg / mL-4.0 mg / mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.

Description

technical field [0001] The present invention relates to biological fermentation and the field of medicine, specifically the present invention relates to the method for producing the culture solution of B. pilosa containing sesquiterpene through the cultivation of B. pilosa, and the nutrient solution or sesquite of B. pilosa obtained by the method The crude terpene, and the pharmaceutical composition and / or health product containing the fermentation broth or the crude sesquiterpene have a drug for preventing and / or treating diabetes, especially it has a hypoglycemic effect, so the culture fluid of Stella pilosa Or the crude sesquiterpene can be used to prepare health products and / or medicines for lowering blood sugar, making it an ideal medicine for lowering blood sugar. Background technique [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger human life and health. The ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A23L1/28A23L1/29A23L33/00
Inventor 张志才王永生
Owner JIANGSU ANHUI BIO TECH
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