Method for producing extracellular polysaccharide through golden fungus liquid fermentation and uses thereof

A technology of exopolysaccharide and liquid fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of species resource limitation, not obvious hypoglycemia, and side effects

Active Publication Date: 2012-08-29
BEIJING LYUKE TIANCHENG BIO TECH
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The existing western medicine hypoglycemic drugs inevitably have side effects, while most of the natural Chi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 golden ear fermented liquid

[0025] 1, in the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experiment Technical Handbook, 1994: P 367), insert the golden ear fungus classification that embodiment 1 obtains, culture temperature 27 ℃, treat that mycelium grows After the slant was full, the slant strains were inserted into 250 mL Erlenmeyer flasks containing 60 mL of shaker flask culture medium (5 bottles in total), and cultured on a shaker at 27° C. and 150 rpm for 24 hours. Wherein the formula of shake flask culture medium is (unit is gram / liter): glucose 5, corn flour 15, soybean meal powder 5, KH 2 PO 4 3. MgSO 4 1. The initial pH value is 6.3.

[0026]2. Insert the above shake flask strains into 500mL Erlenmeyer flasks equipped with 150mL expansion medium (24 flasks in total), the inoculation amount is to insert 11mL shake flask seeds into each 500mL Erlenmeyer flask, and shake at 27°C...

Embodiment 2

[0030] Example 2 Preparation of golden ear fermentation broth and crude exopolysaccharide

[0031] The bacterial classification used in this embodiment is the bacterial classification that embodiment 1 obtains.

[0032] 1. In the newly prepared PDA medium (Zhuge Jian, Wang Zhengxiang, Manual of Industrial Microbiology Experiment Technology, 1994: P367), insert the golden ear fungus species obtained by the tissue separation method, culture at 25°C, and wait for the mycelia to grow fully After the slant, the slant strains were inserted into 250 mL Erlenmeyer flasks (total 3 flasks) equipped with 75 mL of shaking flask culture medium, and cultured on a shaker at 22° C. at 120 rpm for 36 hours.

[0033] 2. Insert the shake flask strain into a 500mL Erlenmeyer flask equipped with 150mL expansion medium (total 10 flasks), the inoculum amount is 15mL shaker flask seeds per 500mL Erlenmeyer flask, 25°C, 120 rpm shaker Incubate for 36 hours.

[0034] 3. Put 1500mL of the expanded cul...

Embodiment 3

[0042] Example 3 Preparation of Fermented Auricularia officinalis Broth and Crude Exopolysaccharide

[0043] The bacterial classification used in this embodiment is the bacterial classification that embodiment 1 obtains.

[0044] 1. In the newly prepared PDA culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P367), insert the golden ear fungus classification that embodiment 1 obtains, culture temperature 25 ℃, treat that mycelium is overgrown After the slant, the slant strains were inserted into 250 mL Erlenmeyer flasks (total 3 flasks) equipped with 75 mL of shaking flask culture medium, and cultured on a shaker at 22° C. at 120 rpm for 36 hours.

[0045] 2. Insert the above shake flask strains into 500mL Erlenmeyer flasks equipped with 150mL expansion medium (total 10 flasks), the inoculum amount is to insert 15mL shaker flask seeds per 500mL Erlenmeyer flask, shake at 25°C and 120 rpm Incubate for 36 hours.

[0046] 3...

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Abstract

The invention relates to the biofermentation field and the medicine field and provides a method for producing exopolysaccharides through the liquid fermentation of golden fungus and a purpose hereof. The invention takes the golden fungus as the starting fungus plant for culture in a liquid shake flask, enlarged culture in the liquid shake flask, first-grade seed culture and fermentation culture to obtain the golden fungus fermentation liquid which contains the exopolysaccharides. The fermentation liquid is obtained after deep fermentation of the golden fungus liquid for hyperfiltration and alcohol precipitation or condensed alcohol precipitation to obtain the crude product of the exopolysaccharides. The total yield of the exopolysaccharides can reach 0.3 to 1 g/l fermentation liquid and the purity is between 30 percent and 60 percent. The exopolysarrcharides obtained with the method can be used for producing medicine or healthcare food which can regulate blood sugar.

Description

technical field [0001] The invention relates to the fields of biological fermentation and medicine, in particular to a method for producing extracellular polysaccharides through liquid fermentation of auricularia auriculae and its application. Background technique [0002] Diabetes is a common and frequently-occurring disease among middle-aged and elderly people. The complications caused by diabetes seriously endanger people's life and health. The existing western medicine hypoglycemic drugs inevitably have side effects, while most of the natural Chinese medicine hypoglycemic drugs do not significantly lower blood sugar, and are limited by species resources. However, the production of hypoglycemic drugs by fermentation of medicinal fungi does not have the above-mentioned disadvantages. [0003] Golden ear is a rare wild rare edible fungus and medicinal fungus. This bacterium belongs to a kind of fungus of Tremella genus, scientific name Tremella aurantialba, grows on the d...

Claims

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Application Information

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IPC IPC(8): C12P19/04A61K31/715A61K36/06A61P3/10A23L1/30C12R1/645
Inventor 张志才黄达明崔凤杰钱静亚肖香管国强吴其飞
Owner BEIJING LYUKE TIANCHENG BIO TECH
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