Cell suspension concentration sensor

A concentration sensor and cell suspension technology, applied in the field of sensors, can solve the problem of inability to directly know the concentration, and achieve the effects of high quality factor Q and frequency stability, fast electrode response and small volume

Inactive Publication Date: 2015-04-15
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When a cell suspension with an unknown concentration is obtained, the existing detection method cannot directly obtain its concentration

Method used

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  • Cell suspension concentration sensor
  • Cell suspension concentration sensor
  • Cell suspension concentration sensor

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Embodiment Construction

[0011] figure 1 Shown is an exploded schematic view of the metal shell, the surface acoustic wave resonator and the screen-printed carbon electrode provided by the present invention. figure 2 Shown is a schematic diagram of the electrode design of the surface acoustic wave resonator provided by the present invention. Please also refer to figure 1 and figure 2 .

[0012] The present invention provides a cell suspension concentration sensor, which includes a metal shell 12 , a surface acoustic wave resonator (SAWR) 11 and a screen-printed carbon electrode 13 . The surface acoustic wave resonator 11 is vacuum-packaged in the metal shell 12. The surface acoustic wave resonator 11 uses ST-cut quartz as the piezoelectric substrate material and is prepared by photolithography. The frequency of the surface acoustic wave emitted is 433MHz. Screen-printed carbon electrodes are connected in series with the surface acoustic wave resonator.

[0013] In the present invention, the sur...

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Abstract

The invention provides a cell suspension concentration sensor, a detecting system and a detecting method thereof. The cell suspension concentration sensor comprises a metal outer shell, an acoustic surface wave resonator and a silk-screen printing carbon electrode, wherein the acoustic surface wave resonator is packaged into the metal outer shell in a vacuum mode; the acoustic surface wave resonator adopts ST (system test) cut quartz as a piezoelectric substrate material, and through the preparation by a photoetching technology, the frequency of emitted acoustic surface wave is 433MHz. The cell suspension concentration sensor provided by the invention is small in volume, practical and convenient, rapid in electrode response and high in flexibility, has the advantages of good bath repeatability, low cost and the like, also can achieve high frequency, and can detect cell suspension concentration to be detected rapidly in real time.

Description

technical field [0001] The invention relates to a sensor, in particular to a cell suspension concentration sensor. Background technique [0002] Cell suspension refers to the digestion and blowing of adherent cells with trypsin, etc., to separate the cells from each other, so that the cells are suspended in the culture medium. There is currently no method for directly measuring the concentration of a cell suspension. Usually, a certain number of cells is taken out, and then the number of cells is observed through a microscope, and then an appropriate amount of solvent is added by calculation to prepare a cell suspension of a certain concentration. To obtain other concentrations of cell suspension, it is necessary to dilute the high-concentration suspension to a low concentration, or concentrate the corresponding low-concentration suspension to a high concentration. When a cell suspension with an unknown concentration is obtained, the existing detection method cannot direct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/06
Inventor 惠国华曾小燕王盈盈童春霞尹芳缘
Owner ZHEJIANG GONGSHANG UNIVERSITY
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