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Extraction method and detection method for berberine and palmatine in cortex phellodendri chinensis

An extraction method and technology for treating Cortex Phellodendri, applied in the field of medicine, can solve problems such as long extraction time, and achieve the effects of shortening extraction time, avoiding structural change loss and low energy consumption

Inactive Publication Date: 2013-06-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention of the present invention is to provide a kind of extracting method of berberine and palmatine in Cortex Phellodendri, solve the problem that extraction time is long in the prior art, provide a kind of rapid detection method of berberine and palmatine in Cortex Phellodendri simultaneously

Method used

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  • Extraction method and detection method for berberine and palmatine in cortex phellodendri chinensis
  • Extraction method and detection method for berberine and palmatine in cortex phellodendri chinensis
  • Extraction method and detection method for berberine and palmatine in cortex phellodendri chinensis

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Experimental program
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Effect test

Embodiment 1

[0022] Different solvent experiments

[0023] The dried Phellodendron officinalis was crushed through a 60-80 mesh sieve, 12 parts of 1.0 g dried Phellodendron officinalis powder were accurately weighed, placed in plastic sealed bags, randomly divided into 4 groups, 3 bags in each group, and 40 ml of water, 70% Ethanol, 70% methanol, 70% acetone, sealed and soaked for 24 hours. Then put it into the ultra-high pressure equipment, pressurize 200 MPa for leaching, extract at 30°C, hold the pressure for 3 min, release the pressure, and after centrifugal filtration, take 1 ml of the extract into a 10 ml volumetric flask, and distill it to volume. The content of berberine and palmatine was determined by HPLC-DAD method. HPLC conditions: Daiso ODS-BP column (5 μm, 4.6 mm × 250 mm); mobile phase: acetonitrile (A)—0.7% phosphoric acid solution (B); gradient elution conditions: 0-5 min, B: 75 %; 5-35 min, B: 60%; column temperature: 30°C; flow rate: 1 ml / min; detection wavelength: 345...

Embodiment 2

[0028] Solvent Concentration Experiment

[0029] The dried Cortex Phellodendron was crushed through a 60-80 mesh sieve, and 12 parts of 1.0 g dried honeysuckle powder were accurately weighed, placed in plastic sealed bags, randomly divided into 4 groups, 3 bags in each group, and 40 ml of 10%, 30%, 50%, 70%, 90% ethanol, sealed and soaked for 24-48 hours. Then put it into the ultra-high pressure equipment, the pressure is 200 MPa, the extraction temperature is 30 ° C, the pressure holding time is 3 min, the pressure is released, and after centrifugal filtration, take 1 ml of the extract in a 10 ml volumetric flask, distill it to volume, and use HPLC- The DAD method was used to determine the content of berberine and palmatine. HPLC conditions were: Daiso ODS-BP column (10 μm, 4.6 mm × 250 mm); mobile phase: acetonitrile (A)—0.7% phosphoric acid aqueous solution (B); gradient elution conditions: 0-5 min, B: 75 %; 5-35 min, B: 60%; column temperature: 30°C; flow rate: 1 ml / min;...

Embodiment 3

[0033] Different extraction pressure comparison test

[0034] Grind the dried Phellodendron officinalis through a 60-80 mesh sieve, accurately weigh 12 parts of 1.0 g dried Phellodendron officinalis powder, put them in plastic sealed bags, divide them into 4 groups randomly, and add 40 ml of 70% ethanol to each group with 3 bags. Seal and soak for 24-48 hours. Then put it into the ultra-high pressure equipment, set the pressure at 100, 200, 300, 400, 500 MPa for leaching, the extraction temperature is 30 °C, the pressure holding time is 3 min, the pressure is released, and after centrifugal filtration, take 1 ml of the extract in 10 ml of volumetric flask, use distilled water to make up volume, adopt HPLC-DAD method to measure the content of berberine and palmatine. HPLC conditions: Daiso ODS-BP column (10 μm, 4.6 mm × 250 mm); mobile phase: acetonitrile (A)—0.7% phosphoric acid aqueous solution (B); gradient elution conditions: 0-5 min, B: 75 %; 5-35 min, B: 60%; column tem...

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Abstract

The invention discloses an extraction method and a detection method for berberine and palmatine in cortex phellodendri chinensis. The extraction method comprises the following steps: crushing dried cortex phellodendri chinensis and carrying out sieving with a 60-mesh to 80-mesh sieve; adding obtained powder in to a solvent according to a solid-liquid mass ratio of 1:10 to 1: 50; soaking the powder for 24 h at a normal temperature after mixing and sealing; placing an obtained mixture in a high pressure vessel and applying a pressure of 100 to 500 MPa at a temperature of 20 to 60 DEG C for extraction, with holdup time being 1 to 5 min; and centrifuging an extractive after pressure relief, taking a supernatant and carrying out filtration so as to obtain an extract of cortex phellodendri chinensis; wherein the solvent is selected from the group consisting of water, ethanol with a concentration of 10 to 90%, methanol with a concentration of 70% and acetone with a concentration of 70%. The supernatant is subjected to HPLC quantitative analysis so as to realize detection. According to the invention, structural changes and loss and reduction in physiological activity of an effective constituent caused by heat effects are avoided, extraction time is short and consumes a small amount of energy, and the detection method is simple, accurate and rapid and has high compactness and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the extraction of effective components in Phellodendron officinalis, in particular to a method for extracting berberine and palmatine from Phellodendron officinalis by using ultra-high pressure. Background technique [0002] Phellodendron Phellodendri is a commonly used Chinese medicinal material, which has the functions of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores. Phellodendron cortex used clinically in traditional Chinese medicine includes two varieties, "Guan Phellodendron" and "Chuan Phellodendron", and the medicinal parts are all dry bark. Before the 2005 edition of "Chinese Pharmacopoeia", Guan Phellodendron and Chuan Phellodendron were recorded together as "Phelloderma Phellodendron". Cortex Phellodendri Phellodendron amurense Rupr. The dry bark of the plant has antibacterial, antipyretic, anti-inflammatory, anti-ca...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 李贺然刘顺
Owner SUZHOU UNIV
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