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A technology of wheat and immature embryos, applied in botany equipment and methods, plant preservation, application, etc., can solve the problems of short time, loss of regeneration potential, limited amount of experimental materials, etc., to reduce the rate of seed germination and prolong the supply time Effect
Inactive Publication Date: 2013-06-19
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
The number of experimental materials derived from artificial environments is limited, especially some scientific research units lack artificial climate facilities, which cannot meet the needs of wheat transformation for immature embryo materials, and wheat immature embryo materials are mainly obtained from the natural environment
However, the time for sampling suitable immature wheat germ materials from the field is very short every year. Under traditional low temperature storage conditions (4°C), they can only be stored for 3 to 5 days, and almost lose their regenerative potential after more than 7 days, which limits the scope of wheat transgenic research. development and productivity
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Embodiment 1
[0018] Example 1. Low-temperature storage test of wheat CB037 immature embryos at -10°C
[0019] In this example, the wheat variety CB037 was taken as the test object, and the effect of tissue culture after the immature embryos of wheat CB037 were stored at -10°C for different periods of time was studied and analyzed. details as follows:
[0020] 1. Acquisition of wheat ears
[0021] The wheat variety CB037 was planted in the experimental farm of the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences (from April to May, the temperature during the wheat growth period was 15-30°C). The immature embryos are heart-shaped embryos with a size of 1.3-1.5 mm.
[0022] 2. Treatment of young ears of wheat at -10°C for different periods of time
[0023] The young ears of wheat CB037 obtained in step 1 were tightly wrapped with tin foil, placed in a plastic bag, and stored in a -10°C low-temperature refrigerator for different periods of time (0, 1, 5, 7, 9,...
Embodiment 2
[0038] Example 2. Low temperature storage test of wheat CB037 immature embryos at -5°C
[0039] In this example, the wheat variety CB037 was used as the test object, and the effect of tissue culture after the immature embryos of wheat CB037 were stored at -5°C for different periods of time was studied and analyzed. details as follows:
[0040] 1. Acquisition of wheat ears
[0041] The wheat variety CB037 was planted in the experimental farm of the Crop Science Research Institute of the Chinese Academy of Agricultural Sciences (from April to May, the temperature during the wheat growth period was 15-30°C). The immature embryos are heart-shaped embryos with a size of 1.3-1.5 mm.
[0042] 2. Treatment of young ears of wheat at -5°C for different periods of time
[0043] Wheat CB037 young ears obtained in step 1 were tightly wrapped with tin foil, placed in a plastic bag, and stored in a -5°C low-temperature refrigerator for different periods of time (0, 5, 10, 15, 20, 25, and ...
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Abstract
The invention discloses a wheat embryo freezing and storing method. According to the wheat embryo freezing and storing method provided by the invention, wheat embryos are stored at the temperature of minus 10 DEG C-0 DEG C. According to the wheat embryo freezing and storing method, immature wheat ears are stored at a low temperature below zero, which prolongs the supply time of the wheat embryos, and solves the problem to a certain extent that the achievement of the wheat embryo is limited by seasonality and artificial climatic conditions, so that the wheat embryo freezing and storing method has an important meaning to the large-scale trangenosis research of the wheat. Experiments show that minus 10 DEG C-0 DEG C is the temperature condition suitable for long-term wheat embryo storage, the wheat embryos can be stored for about 10 days at 10 DEG C, the regeneration rate of the wheat embryos has no significant difference from that of control group wheat embryos which are not stored at low temperature, and the real germination rate is obviously reduced; and the wheat embryos can be stored for about 25 days at -5 DEG C, the regeneration rate of the wheat embryos, especially the regeneration rate of the wheat embryos stored for 5-15 days, is higher than the regeneration rate of the control group wheat embryos which are not stored at low temperature, the generation rate of the wheat embryos stored at -5 DEG C for about 25 days is obviously different from the regeneration rate of the control group wheat embryos, and meanwhile the real germination rate is obviously lowered.
Description
technical field [0001] The invention relates to a method for freezing and storing young wheat embryos. Background technique [0002] Wheat is one of the important food crops in my country, and the use of tissue culture in vitro to obtain regenerated plants is an important link in the development of wheat cell engineering breeding and transgenic breeding. So far, regenerated plants have been obtained by culturing explant materials such as wheat growth point, immature embryo, young panicle, anther, mature embryo, and microspore. However, the regeneration rate varies greatly among different tissues and organs. Among them, wheat immature embryo was considered to be the type of explant with the highest regeneration rate. In order to improve the efficiency of wheat immature embryo culture and regenerated plants, domestic scholars have studied the factors affecting wheat immature embryo culture in detail, including genotype, plant hormones, immature embryo size, medium composition...
Claims
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