Detection method for the Psychrophile content of milk and dairy product

A technology for dairy products and psychrophilic bacteria, applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of small colony growth, inconvenient counting, and long time consumption, and achieve high accuracy, short detection time, The effect of less error

Inactive Publication Date: 2013-06-19
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the detection method of psychrophilic bacteria in milk and dairy products is mainly the national industry standard SN / T 2552.4-2010 "Microbiological test methods for milk and dairy products - Part IV: Colony counting of psychrophilic microorganisms", the first method of which is : The culture condition of the 6.5°C colony counting method is to cultivate at 6.5°C for 10 days, and the detection result is accurate but takes a long time
[0003] In order to solve the time-consuming problem, the above-mentioned standard provides the second method of counting colonies at 21°C. The culture condition adopted is 25±1 hours at 21°C. The colony growth is small within a certain period of time, and the counting is inconvenient, which affects the accuracy of the test results
And because there are other bacteria that can grow besides psychrophilic bacteria under the culture condition of 21°C, not all of the colonies formed in the end are psychrophilic bacteria colonies, so the second method is an approximate detection method, if only for Making the colony grow bigger and prolonging the culture time in the second method will cause more non-psychrophilic bacteria to form new colonies, further affecting the accuracy of the test results
At present, there is still a lack of a method for detecting the content of psychrophilic bacteria in milk and dairy products that can effectively reduce the detection time on the basis of the first method without affecting the detection accuracy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0018] 1. Reagents and samples.

[0019] Use raw milk as the milk or dairy product to be tested.

[0020] Peptone salt solution: commercially available.

[0021] Flat milk count agar: commercially available.

[0022] For the convenience of detection, if the content of bacteria in raw milk is relatively high, it can be diluted in a certain proportion before detection by the method described in this embodiment.

[0023] 2. Operation steps.

[0024] a. Quickly turn over the container containing raw milk to make it evenly mixed; use a sterile pipette to suck 10 mL of raw milk into an Erlenmeyer flask containing 90 mL of sterile peptone salt solution, and shake it enough to obtain a gradient sample T1; another 1 mL sample Bacteria pipette suck 1mL gradient sample T1, add it to the test tube containing 9mL sterile peptone salt solution, shake the test tube, mix well to obtain gradient sample T2; another 1mL sterile pipette suck 1mL gradient sample T2, add to the test tube containing In a tes...

no. 2 example

[0046] 1. Reagents and samples.

[0047] Use raw milk as the milk or dairy product to be tested.

[0048] Normal saline: commercially available

[0049] The total number of colonies test piece uses Beijing Luqiao EASY TEST test piece for the total number of colonies.

[0050] For the convenience of detection, if the content of bacteria in raw milk is relatively high, it can be diluted in a certain proportion before detection by the method described in this embodiment.

[0051] 2. Operation steps.

[0052] a. Quickly turn over the sample container to be tested to make the sample evenly mixed; use a sterile straw to suck 1 mL of raw milk into a triangular flask containing 9 mL of sterile normal saline, and shake it enough to obtain a gradient sample T1; another 1 mL sterile Pipette 1mL gradient sample T1, add it to a test tube containing 9mL sterile saline, shake the test tube, mix well to obtain gradient sample T2; use a 1mL sterile pipette to draw 1mL gradient sample T2, and add it to t...

no. 3 example

[0067] 1. Reagents and samples.

[0068] Use raw milk as the milk or dairy product to be tested.

[0069] Normal saline: commercially available

[0070] The total number of colonies test piece uses Beijing Luqiao EASY TEST test piece for the total number of colonies.

[0071] For the convenience of detection, if the content of bacteria in raw milk is relatively high, it can be diluted in a certain proportion before detection by the method described in this embodiment.

[0072] 2. Operation steps.

[0073] a. Same as described in step a of the operating procedure in the second embodiment.

[0074] b. Same as described in step b of the operating steps in the second embodiment.

[0075] c. Carry out inverted culture of 10 colony cultures. The culture conditions are as follows: first cultivate at 17°C for 19 hours, then at 6.5°C for 4 days; during culture, the colony cultures should not be stacked more than 6.

[0076] d. Same as described in step d of the operating steps in the second embodime...

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PUM

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Abstract

The present invention provides a detection method for the Psychrophile content of milk and dairy products. According to the method, the culture conditions after inoculation are as follows: first incubating at 16-18 DEG C for 18-20 hours, and then incubating at 6-7 DEG C for 4 days. The detection method of the invention costs little time in detection; and the bacterial colonies finally formed is relatively large so errors are not easy to generate during counting. The detection results of the detection method of the invention have a good correlation with the detection results of a 6.5 DEG C colony counting method.

Description

Technical field [0001] The invention relates to a method for detecting microorganisms in milk and dairy products, and is particularly suitable for detecting the content of psychrophilic bacteria in milk and dairy products. Background technique [0002] At present, the detection methods of psychrophilic bacteria in milk and dairy products are mainly based on the national industry standard SN / T 2552.4-2010 "Milk and Dairy Products Hygienic Microbiological Inspection Methods-Part 4: Count of psychrophilic microorganism colonies", of which the first method :The culture condition of the colony counting method at 6.5°C is 10 days at 6.5°C. The detection result is accurate but takes a long time. [0003] In order to solve the time-consuming problem, the above-mentioned standard provides the second method of 21℃ colony counting method. The culture conditions used are 25±1 hours at 21℃. Although the problem of longer time-consuming is solved, the The colony growth is small in time and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 刘晓川喻东威杜丽薛志清刘志楠李梅刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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