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A method for detecting the ability of plants to resist phosphorus deficiency stress by using the characteristics of root organic acid secretion

A feature detection and root exudation technology, which is applied in the direction of measuring devices, material separation, and material analysis, can solve the problems of many parameters, great influence from the external environment, and high instrument cost, and achieve strong plant adaptability, short experiment period, and good quality. The effect of controllability

Inactive Publication Date: 2015-12-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is the most widely used detection method of adversity physiology and stress resistance, but the cost of the instrument is high, the parameters are many and easy to be confused, it is greatly affected by the external environment, and it cannot reflect the long-term changes in the plant.
[0011] There are various shortcomings in the above-mentioned methods, which are not conducive to promotion and popularization in practical applications.

Method used

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  • A method for detecting the ability of plants to resist phosphorus deficiency stress by using the characteristics of root organic acid secretion
  • A method for detecting the ability of plants to resist phosphorus deficiency stress by using the characteristics of root organic acid secretion
  • A method for detecting the ability of plants to resist phosphorus deficiency stress by using the characteristics of root organic acid secretion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The mulberry seedlings were placed in a tray with a bottom, cultured with normal formula Hoagland (Hoagland) nutrient solution for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0044] After culturing for 30 days, the root exudates of two groups of mulberry trees were collected at the same time. Add 200mL, 1mmol / LCaCl 2 The solution is collected in the bottom of the hole tray for 4 hours from 10:00 am to 14:00 pm. The solution in the hole plate is collected and the filtrate obtained by washing the roots of the plants with deionized water is the root exudation of the mulberry tree. Save, ...

Embodiment 2

[0052] The mulberry seedlings were placed in a tray with a bottom, cultured with Hoagland nutrient solution of normal formula for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0053] After culturing for 30 days, the root exudates of two groups of mulberry trees were collected at the same time. Add 200mL, 1mmol / LCaCl 2 The solution is collected in the bottom of the plug tray for 4 hours from 10:00 am to 14:00 pm, the solution in the plug tray is collected and the filtrate obtained by washing the roots of the plants with deionized water is the root exudation of the mulberry tree and stored at low...

Embodiment 3

[0061] The Zhugecai seedlings were placed in a hole tray with a bottom, cultured with a normal formula of Hoagland nutrient solution for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0062] After culturing for 30 days, the root exudates of the two groups of Zhuge Cai were collected at the same time. Add 200mL, 1mmol / LCaCl 2 The solution is placed in the bottom of the plug tray, collected for 4 hours from 10:00 am to 14:00 pm, the solution in the plug tray is collected and the filtrate obtained by rinsing the roots of the plants with deionized water is the root exudate of Zhuge vegetable. Prese...

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Abstract

The invention discloses a method for detecting the ability of plants to resist phosphorus deficiency stress by using the secretion characteristics of organic acids in roots. According to the relational model, the phosphorus extraction capacity of the organic acids secreted by the roots to the rhizosphere soil is calculated, and the ratio of the organic acids secreted by the roots of plants cultivated in the nutrient solution of the phosphorus deficiency formula to the phosphorus extraction capacity of the rhizosphere soil is calculated. The increase ratio of the organic acids secreted by the roots of plants cultured in the nutrient solution of the normal formula to the phosphorus extraction capacity of the rhizosphere soil, and then by calculating the cost of the phosphorus extraction by the organic acids secreted by the roots, the ability of different plants to resist phosphorus deficiency stress can be judged. size. The invention can quantitatively detect the ability of plants to resist phosphorus deficiency stress, the detection results of different plants are comparable, not affected by any time and space geographical factors, and can dynamically monitor the physiological change characteristics of plants for a long time.

Description

technical field [0001] The invention relates to a method for detecting the ability of plants to resist phosphorus deficiency stress by using the secretion characteristics of root system organic acids, and belongs to the field of agricultural engineering technology and the monitoring field of drought resistance ability of crops. Background technique [0002] Phosphorus is one of the important factors limiting plant growth and development. Phosphorus deficiency seriously affects the growth and development of plants. Many studies have shown that phosphorus deficiency stress will increase the secretion of organic acids in plant roots. To activate insoluble phosphorus compounds in the soil such as calcium phosphorus, iron phosphorus and aluminum phosphorus, so as to alleviate phosphorus stress. Therefore, the increase of the content of organic acids secreted by plant roots under phosphorus deficiency stress is a response mechanism to the environmental stress. Microbial activity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 吴沿友赵宽赵玉国邢德科
Owner JIANGSU UNIV
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