Early-stage lung adenocarcinoma miRNA (micro ribonucleic acid) specific expression profile and reverse transcription primer and application thereof

A specific expression profile and lung adenocarcinoma technology, applied in the fields of biology and medical testing, can solve the problems of low sensitivity and specificity in the diagnosis of early lung adenocarcinoma, and achieve a strong clinical effect

Inactive Publication Date: 2013-06-26
SHANGHAI CHEST HOSPITAL
View PDF4 Cites 31 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is that the sensitivity and specificity of early lung adenocarcinoma diagnosis are low. In order to overcome the deficiencies in the prior art, a specific expression profile of early lung adenocarcinoma miRNA and its reverse transcription primer and application are provided.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Early-stage lung adenocarcinoma miRNA (micro ribonucleic acid) specific expression profile and reverse transcription primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention is described in detail below in conjunction with accompanying drawing:

[0020] 1. Collection of serum samples: preoperative peripheral venous blood serum of lung adenocarcinoma patients (AD) and peripheral venous blood serum of healthy examinees (control) whose age and gender matched the experimental group.

[0021] 2. Extraction and purification of serum total RNA:

[0022] 1. Mix 350ul serum samples with an equal volume of 2xDenaturing Solution at room temperature and incubate on ice for 5min.

[0023] 2. Add acid phenol: chloroform, volume = sample volume + 2xDenaturing Solution volume, shake and mix for 30~60s, centrifuge at 12000rpm for 5min, collect the aqueous phase in a clean EP tube.

[0024] 3. Add 1.25 times the volume of 100% ethanol at room temperature to the collected water phase, and mix immediately.

[0025] 4. Add the mixture to the filter, up to 700ul at a time, if the volume is greater than 700ul, the same filter can be used r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of biological and medicine examination, relates to a tumor biomarker, and in particular relates to an early-stage lung adenocarcinoma miRNA (micro ribonucleic acid) specific expression profile and a reverse transcription primer and an application thereof. The early-stage lung adenocarcinoma miRNA specific expression profile comprises more than one of up-regulated expression miR-103, miR-146a, miR-151, miR-221, miR-222 and miR-223. The invention provides an early-stage lung adenocarcinoma diagnosis model, a reverse transcription primer of specific expression miRNA and a kit or biochip for early-stage lung adenocarcinoma diagnosis based on the specific expression profile. The technical scheme provided by the invention can realize accurate diagnosis of early-stage lung adenocarcinoma by detecting a small amount of miRNA, and provides possibility to the production of accurate, efficient and economical detection products. The established lung adenocarcinoma prediction model can distinguish lung adenocarcinoma from a healthy individual, and has great practical significance in clinic.

Description

technical field [0001] The invention belongs to the field of biology and medical examination, and relates to a tumor biomarker, in particular to an early lung adenocarcinoma miRNA-specific expression profile, a reverse transcription primer and use thereof. Background technique [0002] Due to the insidious onset of lung adenocarcinoma, the lack of specific clinical manifestations, and the lack of effective early diagnosis methods, although the 5-year survival rate of stage I lung adenocarcinoma can reach 70%, only 10% of patients can be diagnosed at an early stage. Stage (TIN0M0 / T2N0M0) was found, 40% of lung adenocarcinoma patients have entered the middle and late stages when diagnosed and lost the opportunity for surgery, and the 5-year survival rate was <15%. Therefore, early diagnosis of lung adenocarcinoma is of great significance to improve the quality of life and prolong life of lung adenocarcinoma patients. [0003] Currently recognized methods for early diagnosi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/11C12Q1/68
Inventor 娄加陶薛剑王琳吴传勇
Owner SHANGHAI CHEST HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap