Early-stage lung adenocarcinoma miRNA (micro ribonucleic acid) specific expression profile and reverse transcription primer and application thereof

Abstract

The invention belongs to the field of biological and medicine examination, relates to a tumor biomarker, and in particular relates to an early-stage lung adenocarcinoma miRNA (micro ribonucleic acid) specific expression profile and a reverse transcription primer and an application thereof. The early-stage lung adenocarcinoma miRNA specific expression profile comprises more than one of up-regulated expression miR-103, miR-146a, miR-151, miR-221, miR-222 and miR-223. The invention provides an early-stage lung adenocarcinoma diagnosis model, a reverse transcription primer of specific expression miRNA and a kit or biochip for early-stage lung adenocarcinoma diagnosis based on the specific expression profile. The technical scheme provided by the invention can realize accurate diagnosis of early-stage lung adenocarcinoma by detecting a small amount of miRNA, and provides possibility to the production of accurate, efficient and economical detection products. The established lung adenocarcinoma prediction model can distinguish lung adenocarcinoma from a healthy individual, and has great practical significance in clinic.

Description

technical field [0001] The invention belongs to the field of biology and medical examination, and relates to a tumor biomarker, in particular to an early lung adenocarcinoma miRNA-specific expression profile, a reverse transcription primer and use thereof. Background technique [0002] Due to the insidious onset of lung adenocarcinoma, the lack of specific clinical manifestations, and the lack of effective early diagnosis methods, although the 5-year survival rate of stage I lung adenocarcinoma can reach 70%, only 10% of patients can be diagnosed at an early stage. Stage (TIN0M0 / T2N0M0) was found, 40% of lung adenocarcinoma patients have entered the middle and late stages when diagnosed and lost the opportunity for surgery, and the 5-year survival rate was <15%. Therefore, early diagnosis of lung adenocarcinoma is of great significance to improve the quality of life and prolong life of lung adenocarcinoma patients. [0003] Currently recognized methods for early diagnosi...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is that the sensitivity and specificity of early lung adenocarcinoma diagnosis are low. In order to overcome the deficiencies in the prior art, a specific expression profile of early lung adenocarcinoma miRNA and its reverse transcription primer and application are provided.

Images