Method for early predicting acute cellular rejection after renal transplantation
A technology for rejection and kidney transplantation, applied in the field of transplantation immune detection, can solve the problems of low sensitivity, low specificity and low expression level of immune indicators, and achieve the effects of improving sensitivity, improving high specificity and easy operation.
Inactive Publication Date: 2013-06-26
田普训
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[0002] The traditional method for detecting acute cellular rejection after kidney transplantation is to directly perform ELISA detection on the blood and urine of transplant recipients. The expression level is low, resulting in a small gap in the expression level of immune marker molecules between recipients with normal renal function and acute rejection. Therefore, such traditional methods have the phenomenon of low sensitivity and low specificity of immune indicators detected
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[0044] The present invention will be further described below in conjunction with specific embodiment:
[0045] The invention describes a method for predicting acute cellular rejection after kidney transplantation, and establishes a platform for monitoring immune status after organ transplantation. The present invention separates lymphocytes from the peripheral venous blood of kidney transplant recipients in vitro, stimulates the lymphocytes with PMA and ionomycin for 4-8 hours in vitro, and detects IL-2 and IL-2 in the cell culture supernatant in a flow cytometer. 6. The content of TNF-α and IFN-γ can achieve the purpose of predicting the occurrence of acute cellular rejection.
[0046] Specifically include the following steps:
[0047] 1. In vitro stimulation and flow cytometry detection
[0048] 1) Collect 5ml of heparin-anticoagulated peripheral venous blood from kidney transplant recipients in the morning, centrifuge at 800rpm for 3min, take 0.2ml of plasma and put it in...
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Abstract
The invention discloses a method for early predicting acute cellular rejection after renal transplantation. Lymphocyte is separated from peripheral vein blood of a renal transplantation recipient in vitro after the transplantation and then is stimulated for 4-8 hours in vitro by PMA (polymethyl acrylate) and ionomycin, the content of cell culture supernatant fluids IL-2, IL-6, TNF-alpha and IFN-gamma are detected by a flow cytometry, and the aim of predicting the acute cellular rejection is achieved. The method disclosed by the invention breaks through limitation of low sensitivity and specificity of blood and urine immunity indexes of transplantation recipients in traditional detection, and the sensitivity and specificity of rejection predication are greatly improved. The method is easy to operate, minimally invasive and low in cost, can be used for predicting the acute cellular rejection 3-5 days in advance, and provides an important basis for guiding clinical reasonable use of an immunosuppressor.
Description
technical field [0001] The invention belongs to the field of transplantation immune detection, in particular to a method for early prediction of acute cellular rejection after kidney transplantation. Background technique [0002] The traditional method for detecting acute cellular rejection after kidney transplantation is to directly perform ELISA detection on the blood and urine of transplant recipients. The expression level is low, resulting in a small gap in the expression level of immune marker molecules between recipients with normal renal function and acute rejection. Therefore, such traditional methods have the phenomenon of low sensitivity and low specificity of immune indicators detected. Contents of the invention [0003] The purpose of the present invention is to overcome the technical and functional defects and deficiencies in the field of transplantation immunoassay, aiming at the requirements of high sensitivity and high specificity in the field of transplant...
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IPC IPC(8): G01N15/14
Inventor 田普训靳占奎林远薛武军郑瑾
Owner 田普训