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Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof

A technology for mycoplasma swine pneumonia and inactivated vaccines, which can be applied to bacterial antigen components, respiratory diseases, antibacterial drugs, etc., can solve the problems of difficult promotion and complicated operation of attenuated live vaccines, and achieve good immunogenicity and prevention of mycoplasma hyopneumoniae The effect of disease occurrence and easy operation

Active Publication Date: 2013-07-03
兆丰华生物科技(南京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an inactivated vaccine of Mycoplasma suis pneumonia, which has remarkable immune effect and can effectively prevent the occurrence of Mycoplasma hyopneumoniae disease. promotion problem

Method used

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  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof
  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof
  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of embodiment 1 swine mycoplasma pneumonia inactivated vaccine

[0047] (1) Bacteria (CGMCC No.4545) liquid preparation

[0048] A. Bacterial culture

[0049] Inoculate Mycoplasma hyopneumoniae CGMCC No.4545 bacterium liquid at a ratio of 10% of the medium volume, and culture it at 37±1°C for 5 days.

[0050] B. Pure Test

[0051] According to the current "Chinese Veterinary Pharmacopoeia" appendix for pure test, should be pure.

[0052] C. CCU determination

[0053] Take the harvested bacterial liquid to inoculate the liquid culture medium, and perform a 10-fold serial dilution to 10 -10 , cultured at 37±1°C for 14 days, the titer of live bacteria was 10 10 CCU / ml.

[0054] D. Concentration and purification

[0055] Centrifuge the bacterial solution at 10,000r / min for 30 minutes, discard the supernatant, suspend the precipitated bacterial cells with PBS (pH7.2-7.4) buffer, prepare a bacterial suspension with 1 / 100 of the original culture volume, ...

Embodiment 2

[0072] The preparation of embodiment 2 swine mycoplasma pneumonia inactivated vaccine

[0073] (1) Bacterial solution preparation

[0074] A. Bacterial culture

[0075] Inoculate the Mycoplasma hyopneumoniae CGMCC No.4545 bacterial solution according to 8% of the medium amount, and culture it at 37±1°C for 9 days, and harvest the bacterial solution when the color of the medium turns yellow and slightly turbid, and the pH value drops to 6.50.

[0076] B. Pure Test

[0077] According to the current "Chinese Veterinary Pharmacopoeia" appendix for pure test, should be pure.

[0078] C. CCU determination

[0079] Take the harvested bacterial liquid to inoculate the liquid culture medium, and perform a 10-fold serial dilution to 10 -10 , cultured at 37±1°C for 14 days, the titer of live bacteria was 10 10 CCU / ml.

[0080] D. Concentrate

[0081] Centrifuge the bacterial solution at 10,000r / min for 30 minutes, discard the supernatant, suspend the precipitated bacterial cells w...

Embodiment 3

[0098] Embodiment 3 preparation of swine mycoplasma pneumonia inactivated vaccine

[0099] (1) Preparation of bacterial solution for seedling production

[0100] A. Bacterial culture

[0101] Inoculate the mycoplasma hyopneumoniae CGMCC No.4545 bacterial liquid according to 9% of the medium amount, and culture it at 37±1°C for 7 days.

[0102] B. Pure Test

[0103] According to the current "Chinese Veterinary Pharmacopoeia" appendix for pure test, should be pure.

[0104] C. CCU determination

[0105] Take the harvested bacterial liquid to inoculate the liquid culture medium, and perform a 10-fold serial dilution to 10 -10 , cultured at 37±1°C for 14 days, the titer of live bacteria was 10 11 CCU / ml.

[0106] D. Concentrate

[0107] Centrifuge the bacterial solution at 10,000 r / min for 30 minutes, remove the supernatant, suspend the precipitated bacterial cells with PBS (pH 7.2-7.4) buffer, prepare a bacterial suspension of 1 / 100 of the original culture volume, and stor...

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Abstract

The invention belongs to the technical field of veterinary biological new medicine, and relates to a swine mycoplasma pneumoniae inactivated vaccine and a preparation method thereof. The vaccine adopts a bacterial strain with good immunogenicity, has an immunization persistent period of up to 6 months after intramuscular injection of a single dose of 2.0 ml once, and can effectively prevent the generation of swine mycoplasma pneumoniae diseases. The invention solves the problems of complex operation and difficult popularization of domestic live attenuated vaccines, and fills in the gaps of domestic independent research and development of swine mycoplasma pneumoniae inactivated vaccines.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biomedicine, and relates to an inactivated vaccine of swine mycoplasma pneumonia and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia, also known as swine panting disease or swine endemic pneumonia, is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae. The main clinical symptoms of sick pigs are cough and wheezing, body temperature is basically normal, growth retardation and low feed conversion rate. During necropsy, the main lesions were in the lungs, especially in the heart lobes, middle lobes and apical lobes of the two lungs, which were characterized by pancreatoid changes and fleshy changes. The disease exists all over the world. Although its mortality rate is not high, due to the widespread, long-term and consumption of the epidemic, it can reduce the feed conversion rate and cause a variety of concurrent disease...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P11/00A61P31/04
Inventor 车艳杰王海燕张锋王勇鹣闫国晖高玉梅石松高洁赵亚荣
Owner 兆丰华生物科技(南京)有限公司
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