Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof

A technology for mycoplasma swine pneumonia and inactivated vaccines, which can be applied to bacterial antigen components, respiratory diseases, antibacterial drugs, etc., can solve the problems of difficult promotion and complicated operation of attenuated live vaccines, and achieve good immunogenicity and prevention of mycoplasma hyopneumoniae The effect of disease occurrence and easy operation

Active Publication Date: 2013-07-03
兆丰华生物科技(南京)有限公司 +1
View PDF5 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an inactivated vaccine of Mycoplasma suis pneumonia, which has r

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof
  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof
  • Swine mycoplasma pneumoniae inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Preparation of Inactivated Vaccine against Mycoplasma Swine Pneumonia

[0047] (1) Preparation of bacteria (CGMCC No.4545)

[0048] A. Bacteria culture

[0049] Inoculate the CGMCC No. 4545 bacterial solution of Mycoplasma hyopneumoniae at a rate of 10% of the culture medium, and culture it at 37±1°C for 5 days. The culture medium turns yellow and slightly turbid, and the pH drops to 7.00 to harvest the bacterial solution.

[0050] B. Pure inspection

[0051] Perform pure inspection according to the appendix of the current "Chinese Veterinary Pharmacopoeia" and should be pure.

[0052] C. CCU determination

[0053] Take the harvested bacterial solution to inoculate the liquid medium, and perform a 10-fold serial dilution to 10 -10 , Cultured at 37±1℃ for 14 days, the titer of viable bacteria is 10 10 CCU / ml.

[0054] D. Concentration and purification

[0055] Centrifuge the bacterial solution at 10000r / min for 30 minutes, discard the supernatant, and suspend the pelleted ba...

Example Embodiment

[0072] Example 2 Preparation of Inactivated Vaccine against Mycoplasma Swine Pneumonia

[0073] (1) Preparation of bacterial liquid

[0074] A. Bacteria culture

[0075] Inoculate 8% of the culture medium with CGMCC No. 4545 bacterial solution of Mycoplasma hyopneumoniae, culture at 37±1°C for 9 days, wait until the color of the culture medium turns yellow, becomes slightly turbid, and the pH value drops to 6.50 to harvest the bacterial solution.

[0076] B. Pure inspection

[0077] Perform pure inspection according to the appendix of the current "Chinese Veterinary Pharmacopoeia" and should be pure.

[0078] C. CCU determination

[0079] Take the harvested bacterial solution to inoculate the liquid medium, and perform a 10-fold serial dilution to 10 -10 , Cultured at 37±1℃ for 14 days, the titer of viable bacteria is 10 10 CCU / ml.

[0080] D. Concentration

[0081] Centrifuge the bacterial solution at 10000r / min for 30 minutes, discard the supernatant, and suspend the pelleted bacterial ce...

Example Embodiment

[0098] Example 3 Preparation of Inactivated Vaccine against Mycoplasma Swine Pneumonia

[0099] (1) Preparation of bacterial liquid for seedling production

[0100] A. Bacteria culture

[0101] The culture medium was inoculated with 9% of the amount of the culture medium CGMCC No. 4545 bacterial solution of Mycoplasma hyopneumoniae, cultured at 37 ± 1 ℃ for 7 days, the color of the culture medium turned yellow, slightly turbid, and the pH value dropped to 6.82 to harvest the bacterial solution.

[0102] B. Pure inspection

[0103] Perform pure inspection according to the appendix of the current "Chinese Veterinary Pharmacopoeia" and should be pure.

[0104] C. CCU determination

[0105] Take the harvested bacterial solution to inoculate the liquid medium, and perform a 10-fold serial dilution to 10 -10 , Cultured at 37±1℃ for 14 days, the titer of viable bacteria is 10 11 CCU / ml.

[0106] D. Concentration

[0107] Centrifuge the bacterial solution at 10000 r / min for 30 minutes, remove the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Titeraaaaaaaaaa
Titeraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of veterinary biological new medicine, and relates to a swine mycoplasma pneumoniae inactivated vaccine and a preparation method thereof. The vaccine adopts a bacterial strain with good immunogenicity, has an immunization persistent period of up to 6 months after intramuscular injection of a single dose of 2.0 ml once, and can effectively prevent the generation of swine mycoplasma pneumoniae diseases. The invention solves the problems of complex operation and difficult popularization of domestic live attenuated vaccines, and fills in the gaps of domestic independent research and development of swine mycoplasma pneumoniae inactivated vaccines.

Description

technical field [0001] The invention belongs to the technical field of new veterinary biomedicine, and relates to an inactivated vaccine of swine mycoplasma pneumonia and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia, also known as swine panting disease or swine endemic pneumonia, is a contact chronic respiratory infectious disease caused by Mycoplasma hyopneumoniae. The main clinical symptoms of sick pigs are cough and wheezing, body temperature is basically normal, growth retardation and low feed conversion rate. During necropsy, the main lesions were in the lungs, especially in the heart lobes, middle lobes and apical lobes of the two lungs, which were characterized by pancreatoid changes and fleshy changes. The disease exists all over the world. Although its mortality rate is not high, due to the widespread, long-term and consumption of the epidemic, it can reduce the feed conversion rate and cause a variety of concurrent disease...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/02A61P11/00A61P31/04
Inventor 车艳杰王海燕张锋王勇鹣闫国晖高玉梅石松高洁赵亚荣
Owner 兆丰华生物科技(南京)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap