SNP (Single Nucleotide Polymorphism) marker related to human severe influenza in IFITM3-rs12252 gene and application thereof
An ifitm3-rs12252, severe disease technology, applied in the fields of genetic engineering and molecular biology, can solve problems such as severe influenza that has not yet been found, and achieve the effect of low cost
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Embodiment 1
[0018] Example 1 Obtaining SNP markers associated with human severe influenza
[0019] 1SNP site acquisition and PCR-RFLP typing
[0020] (1) Amplify the nucleotide fragment containing the SNP site
[0021] Primers were designed according to the IFITM3-rs12252 gene sequence (SEQ ID NO.1), including the forward primer F5'-GAAAAGGAAACTGTTGAGAACCGAA-3' and the reverse primer R5'-GAGCCTCCTCCTAAACCTGCAC-3' to amplify the nucleotide of the SNP to be tested fragment. The SNP site is located at 142bp of the IFITM3-rs12252 gene sequence, and the base at this position can be C or T. When the base at this position is T, it can be recognized by MscI endonuclease (the recognition sequence is TGG^CCA).
[0022] Among them, the PCR reaction system is calculated as 50μl: 150-200ng / μl human genomic DNA 1μl, 10μM primers F and R 2μl each, Premix Taq HS 25μl, the balance is ddH 2 O; Among them, Premix Taq HS was purchased from TaKaRa Company (product number: DR028A). Use Premix Taq Hot Start...
Embodiment 2I
[0031] Example 2 Association Analysis and Detection Application of IFITM3-rs12252 Different Genotypes and Human Severe Influenza
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