SNP (Single Nucleotide Polymorphism) marker related to human severe influenza in IFITM3-rs12252 gene and application thereof

An ifitm3-rs12252, severe disease technology, applied in the fields of genetic engineering and molecular biology, can solve problems such as severe influenza that has not yet been found, and achieve the effect of low cost

Inactive Publication Date: 2013-07-10
BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the prediction of severe influenza by detecting a single base mutation

Method used

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  • SNP (Single Nucleotide Polymorphism) marker related to human severe influenza in IFITM3-rs12252 gene and application thereof
  • SNP (Single Nucleotide Polymorphism) marker related to human severe influenza in IFITM3-rs12252 gene and application thereof
  • SNP (Single Nucleotide Polymorphism) marker related to human severe influenza in IFITM3-rs12252 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Obtaining SNP markers associated with human severe influenza

[0019] 1SNP site acquisition and PCR-RFLP typing

[0020] (1) Amplify the nucleotide fragment containing the SNP site

[0021] Primers were designed according to the IFITM3-rs12252 gene sequence (SEQ ID NO.1), including the forward primer F5'-GAAAAGGAAACTGTTGAGAACCGAA-3' and the reverse primer R5'-GAGCCTCCTCCTAAACCTGCAC-3' to amplify the nucleotide of the SNP to be tested fragment. The SNP site is located at 142bp of the IFITM3-rs12252 gene sequence, and the base at this position can be C or T. When the base at this position is T, it can be recognized by MscI endonuclease (the recognition sequence is TGG^CCA).

[0022] Among them, the PCR reaction system is calculated as 50μl: 150-200ng / μl human genomic DNA 1μl, 10μM primers F and R 2μl each, Premix Taq HS 25μl, the balance is ddH 2 O; Among them, Premix Taq HS was purchased from TaKaRa Company (product number: DR028A). Use Premix Taq Hot Start...

Embodiment 2I

[0031] Example 2 Association Analysis and Detection Application of IFITM3-rs12252 Different Genotypes and Human Severe Influenza

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) marker related to human severe influenza and an application thereof. The SNP marker related to human severe influenza is located at 142bp of the sequence of the IFITM3-rs12252 gene shown in SEQ ID NO. 1, and a basic group at 142bp is a C individual, which is at higher risk of suffering from severe influenza than the base group at 142bp, which is a T individual. Polymerase chain reaction (PCR) and sequencing technique are used for screening the SNP marker related to human severe influenza, a restricted fragment length polymorphism (RFLP) method is used for detecting the genotypes of a crowd to be tested, and the early prediction of severe influenza and the control on the generation of severe influenza are possible according to genotype judgment results. In addition, a genotype detecting method provided by the invention avoids the investment of expensive instrument, has the advantages of simpleness, rapidness, low cost and the like and is convenient for popularization and application at a basic level.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a SNP marker in the IFITM3-rs12252 gene related to human severe influenza and its application. Background technique [0002] Influenza (Influenza) is highly contagious and has a high incidence rate, which can easily cause outbreaks and seriously threaten human health. Although influenza vaccine can effectively prevent the occurrence of influenza, the effect of influenza vaccine can be weakened after the mutation of influenza virus. For example, the H1N1 influenza outbreak in 2009, according to statistics, among the 127,000 patients diagnosed in my country, 8,320 were severe and critical cases, and 196 died. It can be seen that influenza, especially severe influenza, has become a major public health problem that threatens human health. [0003] Interferon-induced transmembrane protein 3 (IFITM3), located on human chromosome 11, is a recently discovered p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李宁张永宏董涛赵艳覃岭
Owner BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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