Classification and identification upon 13 pathogenic bacteria by using Fourier transform infrared spectroscopic technology

Abstract

The invention belongs to a classification and identification method upon 13 common pathogenic bacteria, and specifically relates to a method for carrying out classification and identification upon 13 common pathogenic bacteria such as B. cereus, E.coliO157: H7, S.aureus, S.epidermidis, E.coli, E.sakazakii, S.sonnei, L.monocytogenes, C.freunddi, S.typhimurium, E.aerogenes, E.cloacae, and S.enteritidis by using a Fourier transform infrared spectroscopic (FT-IR) technology combined with chemometric operation (hierarchical cluster analysis). The method comprises the steps of the establishment of a FT-IR spectra information database of the 13 pathogenic bacteria, bacteria culturing, ZnSe window manufacturing, spectral acquisition and preprocessing, and result determination by hierarchical cluster analysis. The method has the advantages of high resolution, high speed, simple operation, and low cost.

Description

technical field [0001] The present invention belongs to the rapid classification identification method to 13 kinds of common pathogenic bacteria, specifically uses Fourier transform infrared spectroscopy (Fourier transform infrared spectroscopy, FTIR) technique to Bacillus cereus (B.cereus), Escherichia coli Historian O157:H7 (Escherichia coli O157:H7, E.coli O157:H7), Staphylococcus aureus (S.aureus), Staphylococcus epidermidis (S.epidermidis), Escherichia coli (Escherichia coli , Ecoli), Enterobacter sakazakii (E.sakazakii), Shigella sonnei (S.sonnei), Listeria monocytogenes (L.monocytogenes), Eph Citrobacter freundii (C.freunddi), Salmonella typhimurium (S.typhimurium), Enterobacter aerogenes (E.aerogenes), Enterobacter cloacae (E.cloacae) , Salmonella enteritidis (Salmonella enteritidis, S.enteritidis) for rapid classification and identification. Background technique [0002] Pathogens, especially food-borne pathogens, are an important cause of human diseases and a maj...

AI Technical Summary

Problems solved by technology

However, the application of spectral technology in the classification and identification of microorganisms is not yet systematic and mature enough, and it is not yet standardized enough. It needs to be unified and form a standard method

At present, there is no standardized and unified FT-IR classification and identification method for the above-mentioned 13 pathogenic bacteria at home and abroad, and there is no disclosure of the FT-IT spectral information sharing database for the 13 pathogenic bacteria. This invention can make up for the above defects. Establish a standardized and unified FT-IR classification and identification method for 13 pathogenic bacteria

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