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Oil-degrading composite bacterium and microbial inoculum applied to high salt environment

A technology of petroleum degradation and compound bacterial agent, applied in the field of environmental restoration, can solve the problems of difficult biodegradation of petroleum pollutants, and achieve the effect of fast removal speed, strong adaptability and high activity

Active Publication Date: 2013-08-07
CHINA NAT OFFSHORE OIL CORP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, aiming at the problem that petroleum pollutants are difficult to biodegrade in the current high-salt environment, to provide a kind of petroleum-degrading composite bacteria applied in the high-salt environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Inject Ornithinimicrobium kibberense W25 and Gordonia alkanivorans W33 into beef extract peptone medium (5g beef extract, 10g peptone, 5g NaCl, 1000ml water, pH7.0 (or 7.1 or 7.2), sterilized at 121°C for 20 minutes) , 32 ° C constant temperature shaker 120r / min culture for 72 hours. Centrifuge at 6000r / min for 5 minutes, remove the lower bacterial cell pellet, and wash twice with pH 7.0 (or 7.1, 7.2, 7.3, 7.4 or 7.5) phosphate buffer. The obtained bacteria precipitates were mixed according to the formula in Table 1 to obtain a petroleum-degrading composite bacterium applied in a high-salt environment and a microbial agent of the composite bacterium.

[0044] Table 1. Composition and ratio of the formula (each component is parts by weight)

[0045]

Embodiment 2

[0047] Experimental group: 5g of a compound bacterial agent for petroleum degradation applied in a high-salt environment prepared by formula 6 was mixed evenly with 200g of petroleum-contaminated salinized soil;

[0048] Control group: take 200g of the same oil-polluted salinized soil as the control group;

[0049] The salinity mass concentration of the above-mentioned petroleum-polluted salinized soil is 0.3% (after testing, it contains NaCl0.22%Na 2 SO 4 0.04%); standing in a dark place at room temperature for 20 days, the total petroleum hydrocarbon content in the experimental group decreased from 20.3mg / g to 9.3mg / g, and the petroleum hydrocarbon degradation rate reached 54.2%, while the petroleum hydrocarbon degradation rate in the control group was 12.9%.

Embodiment 3

[0051] Experimental group: 10g of a compound bacterial agent for petroleum degradation applied in a high-salt environment prepared by Formula 7 was mixed evenly with 200g of petroleum-contaminated salinized soil;

[0052] Control group: take 200g of the same oil-polluted salinized soil as the control group;

[0053] The salinity mass concentration of the above-mentioned petroleum-polluted salinized soil is 0.5% (after testing, it contains NaCl0.34%Na 2 SO 4 0.11%); standing in a dark place at room temperature for 20 days, the total petroleum hydrocarbon content in the experimental group decreased from 20.3mg / g to 7.2mg / g, and the petroleum hydrocarbon degradation rate reached 64.5%, while the petroleum hydrocarbon degradation rate in the control group was 15.7%.

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Abstract

The invention discloses an oil-degrading composite bacterium and microbial inoculum applied to a high salt environment. The composite bacterium is composed of a bacterium W25 and a bacterium W33, wherein the bacterium W25 is named Ornithinimicrobium kibberense of which the microbial collection number is CGMCC No.6844; the bacterium W33 is named Gordonia alkanivorans of which the microbial collection number is CGMCC No.6845; and the oil-degrading composite bacterium has the capacity for degrading oil in a high salt environment. The experiment proves that the composite bacterium and microbial inoculum disclosed by the invention have high speed for clearing oil pollution in a high salt environment; the composite bacterium and microbial inoculum disclosed by the invention have wide adaptability to salinity of the oil pollution environment, and can be applied to the high salt environment with the maximum salinity of 8% NaCl; and the composite bacterium and microbial inoculum disclosed by the invention can be used for quickly and effectively treating oil pollution salinized soil, oil pollution seawater, oil pollution wastewater and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental restoration, and relates to a petroleum-degrading bacterial agent applied in a high-salt environment and a preparation method thereof. Background technique [0002] With the continuous enhancement of human oil exploration and development activities, the environment is also bearing a huge risk of oil pollution. A large number of oil pollutants enter the environment through accidents and other ways, causing oil pollution. Oil pollution often occurs in high-saline environments, forming oil-polluted high-saline environments, such as oil-polluted salinized soil, oil-polluted high-salt wastewater, and oil-polluted seawater. Many large oil fields in my country have soil salinization to varying degrees due to their special geographical location. Accidental leakage during oil exploitation can easily cause oil pollution and form oil-polluted salinized soil. In addition, the oil production wastewate...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/10B09C1/10C02F3/34C12R1/01C02F101/32
Inventor 王新新吴亮杨勇安伟牛志刚丁东华
Owner CHINA NAT OFFSHORE OIL CORP
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