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High performance liquid chromatography online analysis method and application thereof

A high-performance liquid chromatography and analysis method technology, applied in the direction of analysis materials, material separation, measurement devices, etc., can solve the problems of inability to online sampling and online analysis, low sensitivity of near-infrared spectroscopy, large initial investment, etc., to reduce work. Real-time display of quantitative and analysis results, and the effect of a high degree of automation

Active Publication Date: 2013-08-07
ZHEJIANG UNIV
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Problems solved by technology

However, near-infrared spectroscopy technology has obvious limitations: first, the sensitivity of near-infrared spectroscopy analysis is relatively low, and its detection limit is only one thousandth; Large upfront investment to build a robust NIR correction model, and its accuracy is strongly related to the quality of model building and the rational use of the model
Chen Ling et al. used solid-phase extraction-liquid chromatography to analyze trace organic matter in water. This online system is only suitable for the enrichment and analysis of trace organic pollutants in drinking water, surface water and other low-viscosity liquids. And it cannot really realize on-line sampling and on-line analysis, nor is it suitable for large-flow samples, and its use is relatively limited

Method used

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  • High performance liquid chromatography online analysis method and application thereof
  • High performance liquid chromatography online analysis method and application thereof
  • High performance liquid chromatography online analysis method and application thereof

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Effect test

Embodiment 1

[0028] 1. See figure 1 , Design a high performance liquid phase online analysis system, which is mainly composed of sample pretreatment system (1), high performance liquid chromatography (2), remote computer (3), programmable logic controller (4) and touch screen (5), The switch (6) is composed, the sample pretreatment system (1) is directly connected to the production process pipeline, and the sample pretreatment system (1) is connected to the high performance liquid chromatograph (2), which can realize automatic sampling and injection of samples; HPLC The chromatograph (2), the remote computer (3) and the touch screen (5) are connected through a switch (6); the programmable logic controller (4) and the touch screen (5) are connected through a network cable (7). The sample pretreatment system (1) consists of a cleaning tank (8), a two-way solenoid valve (9), a cleaning tank three-way solenoid valve (10), a recovery three-way solenoid valve (16), a liquid inlet proportional sol...

Embodiment 2

[0039] The connection method of the liquid chromatography on-line analysis system is the same as that of Example 1, and the system is used to detect the content change of hydroxysafflor yellow A during the safflower extraction process. The process parameters of safflower extraction are as follows: take 1 kg of safflower, add 10 L of pure water, soak and extract at room temperature.

[0040] The operation steps for online detection of safflower extraction process using HPLC online analysis method are as follows:

[0041] 1. The liquid inlet (20) connecting hose of the sample pretreatment system (1) is placed in the extraction tank for real-time sampling, and the liquid outlet (21) of the sample pretreatment system is connected to the six in the high performance liquid chromatograph (2). The e port of the port valve (24) is connected, and the liquid phase recovery port (22) is connected to the f port of the six port valve (24).

[0042] 2. Turn on the high performance liquid chromatog...

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Abstract

The invention provides a high performance liquid chromatography online analysis method. A high performance liquid chromatography online analysis system formed by a sample pretreatment system, a high performance liquid chromatograph, a programmable logic controller, a touch screen and a switch is designed, and is utilized to realize sample pretreatment, liquid chromatography auto-injection analysis, and the real-time treatment and display of the liquid chromatography analysis result. The method has the advantages of high automation degree, simple operation, automatic pretreatment and analysis of a sample, real-time display of the analysis result, realization of the automatic cleaning of the system, and the like, and can be used for the online detection of the sample content and the component proportion in the production process.

Description

Technical field [0001] The invention belongs to the technical field of chromatographic analysis, and specifically relates to an online analysis method of high performance liquid chromatography and its application in the online analysis of the production process of traditional Chinese medicine. Background technique [0002] The quality inspection of the production process of traditional Chinese medicine has recently received great attention from manufacturers, and the near-infrared spectroscopy technology has also been widely used in online inspection of the production process of traditional Chinese medicine because of its fast and non-destructive advantages. However, near-infrared spectroscopy technology has obvious limitations: first, the sensitivity of near-infrared spectroscopy analysis is relatively low, and its detection limit is only one-thousandth; second, near-infrared spectroscopy technology is a secondary analysis method and requires more Large upfront investment is nee...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 吴永江刘雪松丁海樱金叶陈勇栾连军王龙虎
Owner ZHEJIANG UNIV
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