Compound bacterium for leaching of manganese slag
A technology of compound flora and manganese slag, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of high heavy metal content, inability to use economical and environmentally friendly treatment, and low manganese grade.
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[0020] 1. Preparation of complex flora
[0021] Firstly, prepare the culture medium used for the expanded culture of the flora. The formula of the culture medium is as follows: 1%-5% pyrite, 0.05%-0.1% ammonium sulfate and 0.5% potassium dihydrogen phosphate, and the pH is 2.0-3.0. Then, Acidithiobacillus thiooxidans (ATCC No. 8085), A ferrooxidans (ATCC No. 13598), and Leptospirillums ferroxidans (ATCC No. 49879) were inoculated into the culture medium respectively. In the base, 30 ℃, under 180rpm condition, carry out expansion culture, then the bacterial liquid of expansion culture is mixed to make composite flora. Among them, the number ratio of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and Helicobacter leptosus is 1-5:1-5:1, and the number of viable bacteria in the composite flora is 10 7 ~ 10 9 CFU / ml.
[0022] 2. Application of complex flora
[0023] (1) Preparation of biological presoak
[0024] The bacterial culture medium for preparing the mixed bacter...
Embodiment 1
[0032] (1) Preparation of biological presoak
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