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Treatment of spinal cord injury by transplanting stem cells modified by neurotrophic factor gene

A neurotrophic factor and neural stem cell technology, applied in the field of biomedicine, can solve the problems of non-neural tissue, non-proliferative neurons and less integration of host cells.

Inactive Publication Date: 2013-08-21
孙勇
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, primary cultured neurons cannot proliferate in vitro to a sufficient number of neurons for transplantation
Embryonic neural tissue has defects such as difficulty in origin, non-neural tissue, and poor integration with host cells

Method used

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  • Treatment of spinal cord injury by transplanting stem cells modified by neurotrophic factor gene
  • Treatment of spinal cord injury by transplanting stem cells modified by neurotrophic factor gene
  • Treatment of spinal cord injury by transplanting stem cells modified by neurotrophic factor gene

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Embodiment Construction

[0012] (1) Preparation of transgenic neural stem cells (see the invention patent "Construction of embryonic neural stem cells genetically modified by glial cell-derived neurotrophic factor", application number: 201210016282.5), briefly: first construct the target gene GDNF and the retroviral vector pLNCX2 The supernatant of packaging cells pT67 was used to infect embryonic neural stem cells, and the embryonic neural stem cells GDNF-NSC modified by glial cell-derived neurotrophic factor were obtained by G418 selection s .

[0013] (2) Spinal cord injury model production, according to Tator spinal cord injury production method: 200 grams of clean grade female SD rats, 2% pentobarbital sodium (60mg / kg) intraperitoneally anesthetized the animal for about 15-20 minutes, shaved back T8 hair in the vicinity. Disinfect the skin with 75% alcohol, incise the skin and separate the muscles until the spinal canal is completely exposed. Use a rongeur to bite off the T8 spinous process, an...

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Abstract

The invention relates to a technology of biomedicine, concretely to a treatment of a spinal cord injury by transplanting stem cells modified by a neurotrophic factor gene. The treatment comprises: (1) constructing transgenic stem cells, (2) making a spinal cord injury model, (3) performing an inclined plate test, (4) performing an open field test, (5) marking engineering cells, (6) transplanting cells, (7) obtaining organization samples, (8) observing an area of the spinal cord injury, (9) observing survival of the stem cells in vivo, and (10) migrating stem cells in vivo. The transgenic stem cells transplanted to adult rats with the spinal cord injury not only survive and migrate, but also can reduce the area of the spinal cord injury, and promote recovery of neurological function of the rats with the spinal cord injury. Therefore, the transgenic stem cells provide a novel treatment approach for motor neuron injury diseases such as the spinal cord injury.

Description

technical field [0001] The invention relates to a biomedical technology, in particular to a neurotrophic factor gene-modified stem cell transplantation for treating spinal cord injury. technical background [0002] The endogenous repair capacity of the mammalian CNS after injury is limited because it is difficult for the damaged adult CNS to generate new neurons and initiate functional axon regeneration. Although it is possible to induce endogenous CNS stem cells to differentiate into neurons, at the current state of the art, transplantation may be a more feasible approach to repair damaged CNS. Researchers have tried transplanting neural or non-neural cells and applying certain neuroprotective agents in various spinal cord injury (SCI) models to promote axon regeneration and recovery of nerve function. These transplanted tissues or cells include peripheral nerves, embryonic spinal cord tissue, Schwann cells, olfactory nerve sheath cells, activated macrophages and microglia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K38/18A61K35/30A61P25/28
Inventor 孙勇
Owner 孙勇
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