Method for detecting whether quorum sensing system exists in type II bacteriocin producing strain
A quorum sensing system and bacteriocin technology, applied in the field of PCR primer sets, can solve the problems of not forming a set of effective detection methods for bacteriocin quorum sensing regulation, and the detection method not being established.
Active Publication Date: 2013-08-21
CHINA AGRI UNIV
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Problems solved by technology
Quorum sensing is an important mechanism to regulate the synthesis of class II bacteriocins. Lactobacillus producing class II bacteriocins use small peptides as their signaling molecules, but the detection method has not yet been established, which has also become a huge obstacle limiting the research on quorum sensing
Although many class II bacteriocin-producing lactic acid bacteria have been found, whether their bacteriocin production is regulated by quorum sensing has not yet formed an effective detection method
Method used
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Embodiment 1
[0040] Example 1, identification or auxiliary identification of Lactobacillus plantarum (L.paraplantarum) L-XM1 whether there is a quorum sensing system
[0041] 1. Use the method of the present invention to identify or assist in the identification of Lactobacillus plantarum (L.paraplantarum) L-XM1 (the original number is Y1, now it is officially named L.paraplantarum L-XM1 after identification, see: Gui Meng, Zhang Xiaoqiong, in Mu Yang, Wu Ruiyun, Li Pinglan. Screening and identification of heat-resistant lactic acid bacteria natural starter for meat products. Meat Research, 2011, 25(8): 1-5) Is there a quorum sensing system (the experimental process is as follows figure 1 shown).
[0042] The specific operation is as follows:
[0043] 1. Genomic DNA extraction
[0044] Lactobacillus plantarum (L.paraplantarum) L-XM1 was inoculated into MRS medium at an inoculum size of 5% (volume ratio), cultured at 37°C for 24 hours, 2 mL was taken, centrifuged at 13800×g for 1 min, and ...
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Abstract
The invention discloses a PCR primer set used in identifying or auxiliary identifying of whether a quorum sensing system related gene exists in a type II bacteriocin producing strain requiring detection. The PCR primer set is composed of 18 primers respectively composed of DNA single chains represented by Sequence 1, Sequence 2, Sequence 3, Sequence 4, Sequence 5, Sequence 6, Sequence 7, Sequence 8, Sequence 9, Sequence 10, Sequence 11, Sequence 12, Sequence 13, Sequence 14, Sequence 15, Sequence 16, Sequence 17 and Sequence 18 in the sequence list. According to the invention, quorum sensing system three-component gene is amplified through PCR, such that the existence of quorum sensing system is determined. Therefore, large-scale high-flux screening of strains regulated by quorum sensing from type II bacteriocin is realized.
Description
technical field [0001] The invention relates to a PCR primer set for identifying or assisting in identifying whether there are quorum sensing system-related genes in the class II bacteriocin-producing bacteria to be tested, and using the PCR primer set to identify or assist in identifying whether the class II bacteriocin-producing bacteria to be tested are There are methods for quorum sensing systems. Background technique [0002] Quorum Sensing (QS) refers to the phenomenon that the expression of certain genes in microbial populations is regulated by signaling molecules related to population density. The QS system is involved in the regulation of a series of biological functions, such as cell luminescence, biosynthesis of antibiotics, production of virulence factors, synthesis of extracellular polysaccharides, bacterial clustering, biofilm formation, plasmid conjugation transfer, entry into stable growth phase, etc. [0003] The QS system is considered to be involved in th...
Claims
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Login to View More IPC IPC(8): C12Q1/68C12N15/11C12R1/25
Inventor 李平兰张香美赵斌张旭尚楠
Owner CHINA AGRI UNIV