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Application of skimmed milk culture medium in detection of class II bacteriocin producing bacteria QS

A skim milk culture medium, bacteriocin technology, applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problem of not forming a set of effective detection methods for bacteriocins, and achieve the effects of simple and low-cost detection methods.

Active Publication Date: 2015-05-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although many bacteriocin-producing lactic acid bacteria have been found, whether their bacteriocin production is regulated by quorum sensing mechanism has not yet formed an effective detection method

Method used

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  • Application of skimmed milk culture medium in detection of class II bacteriocin producing bacteria QS
  • Application of skimmed milk culture medium in detection of class II bacteriocin producing bacteria QS

Examples

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Embodiment 1

[0039] Example 1. Detection or auxiliary detection of whether there is quorum sensing regulation in the production of class II bacteriocins by Lactobacillus pentosus (L.pentosus) 31-1

[0040] Lactobacillus pentosus 31-1 is a class II bacteriocin producer (Guorong Liu, Yanni Lv, Pinglan Li, Kang Zhou, Jinglan Zhang. Pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31- 1 isolated from Xuan-Wei Ham, a traditional China fermented meat product. Food Control, 2008, 19: 353-359. Zhang Jinlan, Liu Guorong, Shang Nan, Cheng Wanpeng, Chen Shangwu, Li Pinglan. Purification and Partial Amino Acid Sequence of Pentocin 31-1, an Anti-Listeria Bacteriocin Produced by Lactobacillus pentosus 31-1. Journal of Food Protection, Volume 72, Number 12.2524-2529.). This example will detail how to use the method of the present invention to detect or assist in the detection of Lactobacillus pentosus (L.pentosus) 31-1 to produce class II bacteriocins whether there is quorum...

Embodiment 2

[0056] Example 2, detection or auxiliary detection of Lactobacillus pentosus (L.pentosus) LPL1-5 to produce class II bacteriocins whether there is quorum sensing regulation phenomenon

[0057] Lactobacillus pentosus (L.pentosus) LPL1-5 is a type II bacteriocin-producing bacteria (Wu Pengpeng, Liu Guorong, Chang Xiaoyuan, Zhang Xiangmei, Li Pinglan. Purification and characterization of pentosus lactobacillus against Streptococcus suis. China Agriculture University Journal, 2011, 16 (5): 121-126), this embodiment will describe in detail how to utilize the method of the present invention to detect or assist in detecting whether L.pentosus LPL1-5 produces class II bacteriocins and whether there is quorum sensing regulation phenomenon (experimental Process such as figure 1 shown).

[0058] Specifically follow the steps below:

[0059] 1. Preparation of autoinducer (fermentation supernatant)

[0060] Lactobacillus pentosus (L.pentosus) LPL1-5 suspended in sterile physiological sa...

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Abstract

The invention discloses new application of a liquid skimmed milk culture medium. The new application is application in detecting or helping to detect whether the quorum sensing regulation phenomenon exists in class II bacteriocin production by to-be-detected bacteria. The application comprises the following steps: inoculating to-be-detected bacteria having class II bacteriocin production capability into a liquid skimmed milk culture medium, and culturing; in any time of a period before a stationary phase, adding autoinducer into the skimmed milk culture medium, continuously culturing for 50-70 hours to obtain a culture, and detecting whether class II bacteriocins exist in the culture; if so, indicating that the quorum sensing regulation phenomenon exists in class II bacteriocin production by the to-be-detected bacteria or the quorum sensing regulation phenomenon exists in candidates; and if not, indicating that the quorum sensing regulation phenomenon does not exist in class II bacteriocin production by the to-be-detected bacteria or the quorum sensing regulation phenomenon does not exist in the candidates. By using the liquid skimmed milk culture medium in the invention, strains subjected to quorum sensing regulation can be screened from class II bacteriocin producing bacteria in a large-scale and high-flux manner; and the method is simple and convenient, and the cost is low.

Description

technical field [0001] The invention relates to a new application of a liquid skim milk medium, in particular to an application of a liquid skim milk medium in detecting or assisting in detecting whether there is a quorum sensing regulation phenomenon in the production of type II bacteriocins by bacteria to be tested. Background technique [0002] Quorum Sensing (QS) refers to the phenomenon that the expression of certain genes in microbial populations is regulated by signaling molecules related to population density. That is, bacteria synthesize and secrete autoinducer (AI) as a signal molecule, and can sense its concentration change. When the AI ​​concentration reaches a certain threshold with the bacterial population density, specific gene expression is activated to control related biological functions. , such as cell luminescence, biosynthesis of antibiotics, production of virulence factors, synthesis of extracellular polysaccharides, bacterial clustering, biofilm format...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12N1/20C12R1/25C12R1/225
Inventor 李平兰张香美王华王洋
Owner CHINA AGRI UNIV