Continuous microwave assisted proteolysis method

Abstract

The invention discloses a continuous microwave assisted proteolysis method. A device comprises a microwave synthesizer and a low-temperature circulating pump, wherein the microwave synthesizer is an adjustable microwave emission source, a magnetic stirring unit is arranged at the bottom of a microwave shielding cavity of the microwave synthesizer for controlling the magnetic stirring in an enzymatic hydrolysis process, a glass cooling cavity filled with a cooling liquid is formed inside the microwave shielding cavity, a glass enzymatic hydrolysis cavity is formed inside the glass cooling cavity, an infrared temperature probe for monitoring the enzymatic hydrolysis reaction temperature is connected to the center of the glass enzymatic hydrolysis cavity, a snakelike glass condensing tube is coiled outside the glass enzymatic hydrolysis cavity, the low-temperature circulating pump is connected with the snakelike glass condensing tube from the outside of the microwave synthesizer through a pipeline, the cooling liquid is pumped into the snakelike glass condensing tube by the low-temperature circulating pump, and the enzymatic hydrolysis reaction temperature is controlled by regulating the temperature and flow speed of the cooling liquid. The method disclosed by the invention has the advantages that continuous microwave radiation and constant power can be achieved on the premise of stable and unchangeable enzymatic hydrolysis temperature in the enzymatic process so as to improve the enzymatic hydrolysis efficiency and controllability of the enzymatic hydrolysis process.

Description

technical field [0001] The invention belongs to the technical field of microwave-assisted enzyme catalyzed reaction, in particular to a method for continuous microwave-assisted proteolysis. Background technique [0002] Decomposing macromolecular proteins into peptides is an important part of protein research. Through the enzymatic hydrolysis of plant and animal proteins, a series of active peptides with broad application prospects can be developed, such as antihypertensive peptides, antibacterial peptides, immunomodulatory peptides, cell regulatory peptides, antithrombotic peptides, antioxidant peptides and cholesterol-inhibiting peptides Peptides, etc.; traditional enzymatic hydrolysis methods have problems such as long enzymatic hydrolysis time, difficult control of enzymatic hydrolysis conditions, and difficult separation of enzymatic hydrolysis products, enzymes and substrates; microwave-assisted proteolysis technology is a rapid proteolysis technology developed in rece...

AI Technical Summary

Problems solved by technology

Through the enzymatic hydrolysis of plant and animal proteins, a series of active peptides with broad application prospects can be developed, such as antihypertensive peptides, antibacterial peptides, immunomodulatory peptides, cell regulatory peptides, antithrombotic peptides, antioxidant peptides and cholesterol-inhibiting peptides Peptides, etc.; traditional enzymatic hydrolysis methods have problems such as long enzymatic hydrolysis time, difficult control of enzymatic hydrolysis conditions, and difficult separation of enzymatic hydrolysis products, enzymes and substrates; microwave-assisted proteolysis technology is a rapid proteolysis technology developed in recent years. The principle is to use the resonance of microwave frequency to make protein macromolecules oscillate similar to dipole molecules under the action of microwaves. This oscillation makes protein molecules produce coherent and orderly vibrations under the trigger of microwaves, thereby accelerating the enzymatic hydrolysis of proteins. At the same time, microwave has the characteristic of directly penetrating the sample, and can hydrolyze protein into polypeptide or amino acid in a short time, so that the enzymolysis reaction can be carried out quickly and efficiently; It has been widely used; because high temperature often causes protease denaturation and inactivation, the key to microwave-assisted proteolysis technology is to control the sharp rise in temperature caused by the thermal effect of microwave action; the existing microwave-assisted enzymatic The microwave radiation power in the process or the use of intermittent microwave action to achieve the control of the reaction temperature; using these microwave-assisted enzymatic hydrolysis methods, the enzymatic hydrolysis process will become unstable due to the inconstant microwave action power, thus affecting the enzymatic hydrolysis process. The efficiency of control and enzymatic hydrolysis, and at the same time, it is impossible to understand the mechanism of action of protease and protein in the process of microwave-assisted action

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