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Method for reducing chicken breeding flock avian leukosis positive rate

A technology of poultry leukaemia and positive rate, applied in the field of breeding, can solve the problems affecting species conservation, achieve the effect of ensuring population, reducing positive rate, and reducing virus content

Active Publication Date: 2013-09-04
JIANGSU INST OF POULTRY SCI
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AI Technical Summary

Problems solved by technology

[0005] The existing avian leukosis purification method emphasizes that all positive breeding chickens should be strictly eliminated through testing, but this method is not suitable for some core groups with small populations but very high positive rates, especially breeding roosters, usually core groups The number of breeding roosters in the flock is small, and the ratio to the hen flock is 1:10. If the positive rate of the male flock is too high, strict elimination according to the existing method will affect the preservation of the breed, and some chicken breeds are very precious. of

Method used

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Embodiment 1

[0020] Cloacal cotton swabs and fresh whole blood were collected from roosters and hens in the core flock, and the exogenous ALV infection of flocks was detected by ELISA and virus isolation to determine whether each family had a sufficient number of negative roosters for propagation. If the number of negative males is not enough, operate by the following methods;

[0021] 1. Raise the negative hens and roosters separately; mate each negative rooster with 8-12 negative hens during expansion; the negative hens outside the ratio are raised separately for use.

[0022] 2. Positive roosters are raised alone;

[0023] 3. Before multiplication, collect the semen of each positive rooster with a 2ml or more PP conical-bottomed centrifuge tube, mark the rooster number on the PP conical-bottomed centrifuge tube, and add it according to the ratio of semen: saline = 1:1.0-1.5 Sterilize saline and mix well (when the temperature is lower than 10℃, warm the saline to 25℃);

[0024] 4. Cent...

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Abstract

The invention discloses a method for reducing a chicken breeding flock avian leukosis positive rate. Under the condition that the number of negative cocks does not reach a breed conservation requirement, according to a method for processing seminal fluid of positive cocks, under the condition that sperm motility is not influenced, the content of viruses in the seminal fluid of the positive cocks is reduced as far as possible, and then breed mating is carried out on the cocks and the negative hens, namely continuity of flocks is ensured, the positive rate of chicken flocks of a next generation is also effectively reduced, and finally negative core flocks are established after replacement of a few generations.

Description

technical field [0001] The invention belongs to the field of breeding, and in particular relates to a method for reducing the positive rate of avian leukemia in chicken breeding groups. technical background [0002] Avian leukemia is a general term for a variety of neoplastic diseases in birds caused by Avian leucosis virus (ALV), a member of the genus Alpharetrovirus of the family Retroviridae. The disease can be transmitted horizontally or vertically. Congenitally infected immune-tolerant chickens are the most important source of infection. Cross-contamination during breeding is a major way for the pathogen to spread horizontally among chickens. There is no vaccine available for the disease, and flock decontamination is currently the only way to prevent infection. [0003] At present, the commonly used flock purification method is to use ELISA, PCR, virus isolation and other detection technologies to carry out ALV detection on the flocks at different ages in each generati...

Claims

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Application Information

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IPC IPC(8): A01K67/02G01N33/53
Inventor 王克华沈海玉窦套存曲亮窦新红
Owner JIANGSU INST OF POULTRY SCI
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