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Method for reducing positive rate of avian leukemia in chicken breeding flock

A technology of poultry leukaemia and positive rate, applied in the field of breeding, can solve the problems affecting species conservation, achieve the effect of ensuring population, reducing positive rate, and reducing virus content

Active Publication Date: 2015-09-30
JIANGSU INST OF POULTRY SCI
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AI Technical Summary

Problems solved by technology

[0005] The existing avian leukosis purification method emphasizes that all positive breeding chickens should be strictly eliminated through testing, but this method is not suitable for some core groups with small populations but very high positive rates, especially breeding roosters, usually core groups The number of breeding roosters in the flock is small, and the ratio to the hen flock is 1:10. If the positive rate of the male flock is too high, strict elimination according to the existing method will affect the preservation of the breed, and some chicken breeds are very precious. of

Method used

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Embodiment 1

[0020] Collect cloacal swabs and fresh whole blood from core group roosters and hens, and detect exogenous ALV infection in chicken flocks by ELISA and virus isolation methods to determine whether each family has a sufficient number of negative roosters for propagation, If the number of negative cocks is not enough, then operate through the following methods;

[0021] 1. Feed negative hens and negative roosters separately; during multiplication, mate according to the ratio of 8-12 negative hens per negative cock; the negative hens outside the ratio are reared separately and reserved.

[0022] 2. Positive cocks are raised separately;

[0023] 3. Before the multiplication, collect the semen of each positive rooster with more than 2ml of PP centrifuge tubes with conical bottoms and caps, mark the rooster number on the PP centrifuge tubes with conical bottoms and caps, and add according to the ratio of semen: normal saline = 1: 1.0-1.5 Sterilize the saline and mix well (when the ...

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Abstract

The invention discloses a method for reducing a chicken breeding flock avian leukosis positive rate. Under the condition that the number of negative cocks does not reach a breed conservation requirement, according to a method for processing seminal fluid of positive cocks, under the condition that sperm motility is not influenced, the content of viruses in the seminal fluid of the positive cocks is reduced as far as possible, and then breed mating is carried out on the cocks and the negative hens, namely continuity of flocks is ensured, the positive rate of chicken flocks of a next generation is also effectively reduced, and finally negative core flocks are established after replacement of a few generations.

Description

technical field [0001] The invention belongs to the field of breeding, and in particular relates to a method for reducing the positive rate of avian leukemia in chicken breeding flocks. technical background [0002] Avian leukemia is a general term for various tumor diseases in poultry caused by avian leukosis virus (ALV), a member of the genus Alpharetrovirus in the family Retroviridae. The disease can be transmitted horizontally or vertically. Innately infected immune-tolerant chickens are the most important source of infection. Cross-contamination during breeding is a major way for the pathogen to spread horizontally among chicken flocks. There is no vaccine available for the disease and flock decontamination is currently the only way to prevent infection. [0003] At present, the commonly used chicken flock purification method is to use ELISA, PCR, virus isolation and other detection technologies to carry out ALV detection on chicken flocks at different ages in each gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02G01N33/53
Inventor 王克华沈海玉窦套存曲亮窦新红
Owner JIANGSU INST OF POULTRY SCI
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