Jinghai yellow chicken 16-week weight molecular genetic marker and application thereof

A technology of molecular genetic markers and sea yellow, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of long breeding cycle and high cost

Inactive Publication Date: 2013-09-04
YANGZHOU UNIV
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AI Technical Summary

Problems solved by technology

[0002] Although the conventional breeding method has made some progress in the genetic improvement of poultry, its selection of quan

Method used

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  • Jinghai yellow chicken 16-week weight molecular genetic marker and application thereof
  • Jinghai yellow chicken 16-week weight molecular genetic marker and application thereof
  • Jinghai yellow chicken 16-week weight molecular genetic marker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0015] 1. Test material

[0016] Blood samples from 200 Jinghai yellow chickens were collected from Jiangsu Jinghai Poultry Industry Group Co., Ltd., 1.5mL blood samples were collected from the wing vein, anticoagulated with sodium heparin, and stored at -20°C. Genomic DNA was extracted by phenol-chloroform extraction, dissolved in TE, and the concentration of genomic DNA was measured for later use.

[0017] 2. Primer Design and PCR Amplification

[0018] 2.1 Design of SSCP primers

[0019] According to the chicken IGFBP-1 gene exon 2 sequence published in GenBank (accession number: NC_006089), IGFBP-2 gene third intron sequence (accession number: NC_006094), STAT5b gene intron 13 sequence ( Accession number: NC_006114) designed a pair of primers. The sizes of the amplified products were 192, 250 and 272bp, respectively. The primer sequences are as follows:

[0020] IGFBP-1: F: 5'-CCAGAATCTACCGAGCCTGA-3' (SEQ ID NO: 1)

[0021] R: 5'-CCCACTCACCTGTTCTTTCC-3'...

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Abstract

The invention relates to the field of poultry breeding, particularly application of a Jinghai yellow chicken 16-week weight molecular genetic marker. The molecular marker is composed of genes IGFBP-1, IGFBP-2 and STAT5b. The screening method comprises the following steps: extracting chicken genome DNA (deoxyribonucleic acid) of a sample to be detected, amplifying with specific primers disclosed as SEQ ID NO:1-6 to obtain 192bp, 250bp and 270bp target segments, carrying out SSCP analysis on the target segments, carrying out gel electrophoresis, carrying out color development by silver staining to obtain DNA SSCP spectra, and selecting an individual, of which the genotypes of the genes IGFBP-1, IGFBP-2 and STAT5b are the BB/KL/OP three-gene composite genotype, according to the banding patterns in the 3 spectra. The method provided by the invention has the advantages of obvious effect and simple and quick detection, and is free from the influence of the external environment.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for increasing the body weight of chickens on the market by utilizing the joint effect of three genes. Background technique [0002] Although the conventional breeding method has made some progress in the genetic improvement of poultry, its selection of quantitative traits requires a certain period of individual growth and development, and the breeding cycle is long and the cost is high. Most of the growth traits of chickens are quantitative traits controlled by polygenes, and the performance value is the result of the interaction of internal polygenes and the external environment. If molecular assisted breeding is carried out from multiple genes, it will not only speed up the breeding process, It can also comprehensively improve the growth performance of chickens. Many research results have shown that there are multiple genetic loci that affect the growth traits of chic...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 王金玉张跟喜赵秀华施会强俞亚波顾玉萍戴国俊谢恺舟
Owner YANGZHOU UNIV
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