Novel mixed type cornea and conjuctiva intravital staining agent

A living staining and hybrid technology, which is applied to preparations and pharmaceutical formulations for in vivo experiments, can solve the problem that corneal epithelial cells and ocular surface epithelial cells cannot be checked for death or decay at the same time, so as to improve the efficiency of clinical diagnosis and avoid Cross infection, less irritating effect

Inactive Publication Date: 2013-09-18
TIANJIN JINGMING NEW TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current ocular surface stains, Lissamine Green and Fluorescein Sodium, are used alone to stain the cornea and conjunctiva respectively, and can

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Weigh the physiological buffer solution prepared by sodium fluorescein and water for injection, and prepare a sodium fluorescein aqueous solution with a concentration of 0.5%;

[0026] (2) Weighing the physiological buffer solution prepared by Lissamine Green and water for injection to prepare Lissamine Green aqueous solution with a concentration of 0.5%;

[0027] (3) Prepare a mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution according to the volume ratio of fluorescein sodium and lissamine green 1:0.25;

[0028] (4) Filter the mixed solution prepared in step (3) with a filter having a pore size of 0.22u.

[0029] (5) Soak the filter paper with the mixed solution prepared in step (4) to make a dyed test strip, pack it separately, and sterilize it for one-time use.

Embodiment 2

[0031] (1) Take sodium fluorescein and water for injection to prepare a sodium fluorescein aqueous solution with a concentration of 2.0%;

[0032] (2) Weigh Lissamine Green and water for injection to prepare a 2.0% Lissamine Green aqueous solution;

[0033] (3) Prepare a mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution according to the volume ratio of fluorescein sodium and lissamine green 1:1.5;

[0034] (4) Filter the mixed solution prepared in step (3) with a filter having a pore size of 0.22u.

[0035] (5) Soak the filter paper with the mixed solution prepared in step (4) to make a dyed test strip, pack it separately, and sterilize it for one-time use.

Embodiment 3

[0037] (1) Weigh fluorescein sodium and water for injection to prepare a 4.0% sodium fluorescein aqueous solution;

[0038] (2) Weigh Lissamine Green and water for injection to prepare a 4.0% Lissamine Green aqueous solution;

[0039] (3) Prepare a mixed solution of fluorescein sodium aqueous solution and lissamine green aqueous solution according to the volume ratio of fluorescein sodium and lissamine green at a volume ratio of 1:3;

[0040] (4) Filter the mixed solution prepared in step (3) with a filter with a pore size of 0.22u, divide it into disposable containers, and then use it.

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PUM

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Abstract

The invention relates to a novel mixed type cornea and conjuctiva intravital staining agent. A staining agent comprises a mixed solution of a fluorescein sodium aqueous solution and a lissamine green aqueous solution. The mixed type cornea and conjuctiva intravital staining agent has the advantages that the staining agent can stain cornea and bulbar conjunctiva, stains at one time, has small stimulus to eye tissues and is easily accepted by patients; the staining agent is mainly used for early diagnosing and evaluating dry eye syndrome, keratohelcosis, keratoconjunctivitis (KCS), arborization corneal epithelium herpes, Sjogren syndrome and the like.

Description

technical field [0001] The invention relates to a live staining agent for cornea and conjunctiva, in particular to a new mixed type live staining agent for cornea and conjunctiva. In particular, it relates to a staining agent that can directly stain the cornea and conjunctiva, and is clinically used for diagnosing damage or pathology of the cornea and conjunctiva. The staining agent can be prepared in the form of a test strip by infiltrating filter paper. Background technique [0002] Ocular surface staining is a convenient and effective method for diagnosing ocular surface diseases (especially ocular surface damage caused by dry eye disease). Staining of the ocular surface is used to assess the integrity of the epithelial cells of the cornea and conjunctiva because the staining can indicate damage to the epithelium and cell junctions. Sodium fluorescein, tiger red, and lissamine green are commonly used vital stains on the ocular surface. Sodium fluorescein is the primary ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 肖光礼吴亮
Owner TIANJIN JINGMING NEW TECH DEV CO LTD
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