Microbial agent and preparation method as well as application thereof

A technology for microbial inoculants and seeds, applied to microbial inoculants and their preparation methods and application fields, can solve the problems of incomplete fiber degradation, high requirements for composting equipment, poor compost quality, etc., and achieves simple composition and structure of bacteria and good prevention and control. Effect, the effect of fast fermentation rate

Active Publication Date: 2014-11-19
南京宁粮生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The cellulose in crop straws mostly forms dense crystallization regions, and is embedded in hemicellulose and lignin at the same time, making it difficult to enzymatically decompose. Therefore, the natural composting process of straws has long composting time, large nutrient loss, and incomplete composting of fiber degradation. There are many problems such as poor quality. At present, there are many kinds of bacterial agents used to accelerate the composting process and improve the composting effect at home and abroad. The bacterial agent used for composting cellulosic waste has the characteristics of a large number of bacterial species, strong specificity of fermented materials and high requirements for composting equipment, resulting in high composting costs. The patent with publication number CN101486597A provides a composite fermentation bacterial agent , the bacteria agent was added to the bacteria agent 1# and the bacteria agent 2# in the early and late stages of composting, which not only realized the rapid decomposition of the cellulose material, but also improved the nutrient fixation rate, but the bacteria agent 1# and the bacteria agent 2# contained a total of 13 strains of functional bacteria, the production of bacterial agents requires a large workload and high cost; the patent with publication number CN1186441C provides a bacterial agent that decomposes straw into fertilizer, and the bacterial agent is composed of 10 strains of functional bacteria; the patent with publication number CN1680218A Provided is an organic fertilizer fermentation bacterial agent composed of 5 strains of bacteria, the main composting materials of which are bamboo shoot skin and young bamboo; the patent with the publication number of CN101701192A provides a compost bacterial agent with only one high-temperature fungus, whose compost The raw material is tobacco; the patent with publication number CN102010824A provides a bacterial agent for crop straw composting, but when the bacterial agent is used for composting, forced oxygen supply is required, and a solid fermentation tank is required, which increases the cost of composting

Method used

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  • Microbial agent and preparation method as well as application thereof
  • Microbial agent and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 bacterial agent

[0026] 1) Fermentation of Bacillus subtilis and Bacillus megaterium

[0027] LB medium: yeast powder 5 g / L, peptone 10 g / L, NaCl 10 g / L, pH 7.0, sterilized at 121°C for 25 min.

[0028] The fermentation medium involved in the seed tank and fermenter: glucose 5 g / L, peptone 10 g / L, NaCl 10 g / L, pH 7.0, sterilized at 121°C for 25 min.

[0029] Inoculate the slant bacteria stored at 4°C in a 250 mL shake flask filled with 100 mL LB medium, activate and culture at 35°C, 170 r / min for 24 h, and then inoculate the activated bacterial solution with 10% (v / v) The inoculum was transferred into a 1000 mL shake flask filled with 500 mL LB medium, and cultured at 35 °C and 170 r / min for 12 h to obtain a seed solution.

[0030] Put the above seed liquid into the fermentation medium in the seed tank at the inoculum amount of 10% (v / v), ferment and cultivate at 35°C and 180 r / min for 24 h, ferment the seed liquid, and then inoculate ...

Embodiment 2

[0040] The preparation of embodiment 2 bacterial agents

[0041] The preparation of bacterium and fungal fermentation broth is identical to 1 and 2 in embodiment 1, the fermentation broth of Bacillus subtilis, Bacillus megaterium, Trichoderma longibranchus and Aspergillus niger is in mass ratio 1: 1: 1: 1 Mix evenly, then adsorb on vermiculite according to the mass ratio of 1:5, and then dry at 35~40°C until the water content is lower than 15%, and it will be ready after testing according to the standard of agricultural microbial agent (GB 20287-2006). Obtain the bacterial agent.

Embodiment 3

[0042] Example 3 Test of composting wheat straw with microbial inoculum

[0043] Crush the wheat straw into straw sections of 10-15 cm, and add the microbial agent prepared in Example 1 at a mass ratio of 2%. Mix the soil, and then mix the mixture and straw evenly, adjust the water content of the pile to about 55-65%, and pile the compost into strips according to the length, width and height of 2 m×1.5 m×1 m. After composting for about 2 days, the temperature When the temperature rises to 60°C, the piles are turned over to supply oxygen in sequence. The piles are turned once every 8 days, and the piles are turned twice in total. The body volume is reduced to about 1 / 2 of the original, that is, high-efficiency wheat straw organic fertilizer.

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Abstract

The invention relates to a microbial agent and a preparation method as well as an application of the microbial agent. The microbial agent comprises functional bacteria and an adsorbent and is characterized in that the functional bacteria comprise bacillus subtilis, bacillus megatherium, trichoderma longibrachiatum and aspergillus niger, wherein the mass ratio of the microbial fermentations of the bacillus subtilis, the bacillus megatherium, the trichoderma longibrachiatum and the aspergillus niger in a mixture is 0.5-2:0.5-2:0.5-2:0.5-2. The microbial agent is prepared by carrying out the processes of slant culture, shake-flask culture, seeding tank fermentation, fermentation tank fermentation, microbial fermentation mixing, absorbent absorption, and low-temperature drying, and the microbial agent can be applied to the production of straw manure.

Description

technical field [0001] The invention relates to a microbial agent and a preparation method and application thereof, belonging to the field of biotechnology, in particular to a microbial agent capable of promoting rapid composting of crop stalks such as wheat straw, rice straw and corn stalks and a preparation method thereof. technical background [0002] my country is a large agricultural country and one of the countries with the most abundant crop straw in the world. The annual straw production is about 700 million tons, and with the increase of crop yield, the straw production will further increase. A large amount of straw is discarded or burned, which is not only a waste of resources, but also brings huge environmental pollution and ecological damage, seriously endangering people's health. [0003] On the other hand, my country has a large population and a shortage of cultivated land resources, and the multi-cropping index of farmland is high. The resulting depletion of so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/00
CPCY02W30/40
Inventor 梁晓辉何建桥常志州黄红英徐茂管永祥陈光亚王绪奎殷广德梁永红吴昊孔国顺马宏卫戴波吴息正梅成兵邵孝侯俞禄窦培冲赵敏
Owner 南京宁粮生物工程有限公司
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