Multiple rapid detection method of two food-borne pathogenic bacteria and detection primer groups as well as kit

Abstract

The invention discloses a multiple rapid detection method of two food-borne pathogenic bacteria and detection primer groups as well as a kit, wherein the sequences of the upstream and downstream primers of an outer primer and the upstream and downstream primers of an inner primer in a quick detection primer group for salmonella are as shown in SEQ No.1, SEQ No.2, SEQ No.3 and SEQ No.4, respectively; and the sequences of the upstream and downstream primers of an outer primer and the upstream and downstream primers of an inner primer in a quick detection primer group for staphylococcus aureus, and the sequence of a loop primer for improving amplification efficiency are as shown in SEQ No.5, SEQ No.6, SEQ No.7, SEQ No.8 and SEQ No.9. The multiple rapid detection method provided by the invention realizes rapid detection of the DNAs of salmonella and staphylococcus aureus in the same system and under the same reaction conditions by using multiple LAMP detection technology in combination with a melting curve analysis method.

Description

technical field [0001] The invention belongs to the field of molecular biology detection of food microorganisms, and relates to a multiple rapid detection method for Salmonella and Staphylococcus aureus, a detection primer set and a detection kit. Background technique [0002] Food safety is a major concern of all countries in the world, and food-borne pathogens are one of the main reasons affecting food safety. According to the current national food safety supervision system, both Salmonella and Staphylococcus aureus are food-borne pathogens that are of great concern. Routine detection adopts traditional culture methods, and each pathogenic bacteria needs to be tested separately with an independent detection method. The workload is heavy, the detection cycle is long, and it is limited by various factors such as the professionalism and experience of the testing personnel, which is prone to misjudgment. With the gradual application of molecular biology methods in the detecti...

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Problems solved by technology

In addition, because the LAMP amplification product presents a ladder-like band in ordinary electrophoresis, multiple LAMP products cannot be identified by ordinary electrophoresis to distinguish the molecular weight of the target product. In previous reports, more tedious enzyme digestion or sequencing methods were often used. The method to identify the amplified product, the analysis and identification of the amplified product still needs to be improved

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